{"id":1004,"date":"2017-02-27T21:19:33","date_gmt":"2017-02-27T21:19:33","guid":{"rendered":"https:\/\/www.progeriaresearch.org\/?page_id=1004"},"modified":"2025-02-11T09:34:37","modified_gmt":"2025-02-11T14:34:37","slug":"lymphoblast-cell-culture-protocols","status":"publish","type":"page","link":"https:\/\/www.progeriaresearch.org\/ko\/lymphoblast-cell-culture-protocols\/","title":{"rendered":"\ub9bc\ud504\ubaa8\uc138\ud3ec \ubc30\uc591 \ud504\ub85c\ud1a0\ucf5c"},"content":{"rendered":"<p>[et_pb_section fb_built=&quot;1&quot; \uc804\uccb4 \ub108\ube44=&quot;\ucf1c\uc9d0&quot; \ube44\ud65c\uc131\ud654\ub428_\ucf1c\uc9d0=&quot;\uaebc\uc9d0|\uaebc\uc9d0|\uaebc\uc9d0&quot; _\ube4c\ub354_\ubc84\uc804=&quot;4.16&quot; \ud14c\ub450\ub9ac_\ub108\ube44_\ubc14\ub2e5=&quot;55px&quot; \ud14c\ub450\ub9ac_\uc0c9\uc0c1_\ubc14\ub2e5=&quot;#29327a&quot; \uc7a0\uae40=&quot;\uaebc\uc9d0&quot; \uae00\ub85c\ubc8c_\uc0c9\uc0c1_\uc815\ubcf4=&quot;{}&quot;][et_pb_\uc804\uccb4 \ub108\ube44_\ud5e4\ub354 _\ube4c\ub354_\ubc84\uc804=&quot;4.16&quot; \uc81c\ubaa9_\uae00\uaf34=&quot;|||||||||&quot; \uc81c\ubaa9_\uae00\uaf34_\ud06c\uae30=&quot;55&quot; \ubc30\uacbd_\uc0c9\uc0c1=&quot;#29327a&quot; \ubc30\uacbd_\uc774\ubbf8\uc9c0=&quot;https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/12\/Jes.jpg&quot; \ubc30\uacbd_\uc704\uce58=&quot;center_left&quot; \uc0ac\uc6a9\uc790 \uc815\uc758_\ud328\ub529=&quot;9vw||9vw||true&quot; \uc0ac\uc6a9\uc790 \uc815\uc758_\ud328\ub529_\ud0dc\ube14\ub9bf=&quot;&quot; \uc0ac\uc6a9\uc790 \uc815\uc758_\ud328\ub529_\ud734\ub300\ud3f0=&quot;|56px||&quot; \uc0ac\uc6a9\uc790 \uc815\uc758_\ud328\ub529_\ub9c8\uc9c0\ub9c9_\ud3b8\uc9d1=&quot;\ub370\uc2a4\ud06c\ud1b1\uc5d0\uc11c&quot; \uc81c\ubaa9_\uae00\uaf34_\ud06c\uae30_\ud0dc\ube14\ub9bf=&quot;45px&quot; \uc81c\ubaa9_\uae00\uaf34_\ud06c\uae30_\ud734\ub300\ud3f0=&quot;40px&quot; \uc81c\ubaa9_\uae00\uaf34_\ud06c\uae30_\ub9c8\uc9c0\ub9c9_\ud3b8\uc9d1=&quot;\ud734\ub300\ud3f0\uc5d0\uc11c&quot; z_index_tablet=&quot;500&quot; \uc0ac\uc6a9\uc790 \uc815\uc758_css_main_element=&quot;\ubc30\uacbd-\uc704\uce58: \uac00\uc6b4\ub370 18% !\uc911\uc694;&quot; global_colors_info=&quot;{}&quot;]<\/p>\n<h1 class=\"vc_custom_heading\" data-fontsize=\"34\" data-lineheight=\"48\" style=\"text-align: right;\">\ub9bc\ud504\ubaa8\uc138\ud3ec<\/h1>\n<h1 class=\"vc_custom_heading\" data-fontsize=\"34\" data-lineheight=\"48\" style=\"text-align: right;\">\ubb38\ud654 \ud504\ub85c\ud1a0\ucf5c<\/h1>\n<p>&nbsp;<\/p>\n<p>[\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=&quot;1&quot; use_custom_gutter=&quot;on&quot; gutter_width=&quot;1&quot; specialty=&quot;on&quot; padding_left_1=&quot;35px&quot; padding_left_2=&quot;35px&quot; padding_2_tablet=&quot;|||0px&quot; padding_2_phone=&quot;&quot; padding_2_last_edited=&quot;on|desktop&quot; module_class_1=&quot;sidebar-secondary-nav&quot; module_class=&quot;handprint-bg&quot; _builder_version=&quot;4.16&quot; background_image=&quot;https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2019\/04\/blue-handprint-only.png&quot; parallax=&quot;on&quot; parallax_method=&quot;off&quot; inner_width=&quot;100%&quot; inner_max_width=&quot;100%&quot; \uc0ac\uc6a9\uc790 \uc815\uc758 \ud328\ub529=&quot;0|0px|54px|0px|false|false&quot; z_index_tablet=&quot;500&quot; border_width_top=&quot;10px&quot; border_color_top=&quot;#8fd2ed&quot; use_custom_width=&quot;on&quot; width_unit=&quot;off&quot; custom_width_percent=&quot;100%&quot; global_colors_info=&quot;{}&quot;][et_pb_column type=&quot;1_4&quot; _builder_version=&quot;4.16&quot; \uc0ac\uc6a9\uc790 \uc815\uc758 \ud328\ub529=&quot;|||&quot; global_colors_info=&quot;{}&quot; custom_padding__hover=&quot;|||&quot;][et_pb_sidebar area=&quot;et_pb_widget_area_14&quot; disabled_on=&quot;on|on|off&quot; module_class=&quot;subpage-sidebars&quot; _builder_version=&quot;4.16&quot; animation_style=&quot;fade&quot; z_index_tablet=&quot;500&quot; border_width_right=&quot;5px&quot; locked=&quot;off&quot; global_colors_info=&quot;{}&quot;]<br \/>\n[\/et_pb_sidebar][\/et_pb_column][et_pb_column type=&quot;3_4&quot; specialty_columns=&quot;3&quot; _builder_version=&quot;4.16&quot; custom_padding=&quot;|||&quot; global_colors_info=&quot;{}&quot; custom_padding__hover=&quot;|||&quot;][et_pb_row_inner _builder_version=&quot;4.16&quot; background_color=&quot;#00b2e2&quot; custom_margin=&quot;41px||&quot; custom_margin_tablet=&quot;0px||&quot; custom_margin_phone=&quot;&quot; custom_margin_last_edited=&quot;on|phone&quot; custom_padding=&quot;39.4375px|20px|0|20px|false|true&quot; animation_style=&quot;slide&quot; animation_direction=&quot;top&quot; animation_intensity_slide=&quot;25%&quot; box_shadow_style=&quot;preset1&quot; box_shadow_blur=&quot;38px&quot; box_shadow_spread=&quot;-12px&quot; locked=&quot;off&quot; global_colors_info=&quot;{}&quot;][et_pb_column_inner saved_specialty_column_type=&quot;3_4&quot; _builder_version=&quot;4.16&quot; custom_padding=&quot;|||&quot; global_colors_info=&quot;{}&quot; custom_padding__hover=&quot;|||&quot;][et_pb_text admin_label=&quot;\ud504\ub85c\uac8c\ub9ac\uc544 \uc5f0\uad6c \uc7ac\ub2e8\uc758 \uc791\uc5c5...&quot; _builder_version=&quot;4.16&quot; header_2_line_height=&quot;1.2em&quot; background_layout=&quot;dark&quot; custom_margin=&quot;||35px&quot; custom_padding=&quot;|50px||&quot; custom_padding_tablet=&quot;|50px||50px||true&quot; custom_padding_phone=&quot;&quot; custom_padding_last_edited=&quot;on|\ub370\uc2a4\ud06c\ud0d1&quot; header_font_size_tablet=&quot;&quot; header_font_size_phone=&quot;&quot; header_font_size_last_edited=&quot;on|\ub370\uc2a4\ud06c\ud0d1&quot; header_2_font_size_tablet=&quot;25px&quot; header_2_font_size_phone=&quot;&quot; header_2_font_size_last_edited=&quot;on|\ud0dc\ube14\ub9bf&quot; header_4_font_size_tablet=&quot;&quot; header_4_font_size_phone=&quot;19px&quot; header_4_font_size_last_edited=&quot;on|\ud734\ub300\ud3f0&quot; header_4_line_height_tablet=&quot;&quot; header_4_line_height_phone=&quot;1.2em&quot; header_4_line_height_last_edited=&quot;on|\ud734\ub300\ud3f0&quot; z_index_tablet=&quot;500&quot; \uae00\ub85c\ubc8c_\uc0c9\uc0c1_\uc815\ubcf4=&quot;{}&quot;]<\/p>\n<h2 style=\"text-align: center;\"><strong>\ud615\uc9c8\uc804\ud658 \ub9bc\ud504\uad6c \ubc30\uc591 \ud504\ub85c\ud1a0\ucf5c<\/strong><\/h2>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner custom_padding_last_edited=\u201don|phone\u201d _builder_version=\u201d4.16\u2033 custom_padding=\u201d|35px|0|0px|false|false\u201d custom_padding_tablet=\u201d|35px||35px||true\u201d custom_padding_phone=\u201d\u201d animation_direction=\u201dtop\u201d global_colors_info=\u201d{}\u201d][et_pb_column_inner saved_specialty_column_type=\u201d3_4\u2033 _builder_version=\u201d4.16\u2033 custom_padding=\u201d|||\u201d global_colors_info=\u201d{}\u201d custom_padding__hover=\u201d|||\u201d][et_pb_text _builder_version=\u201d4.27.4\u2033 background_size=\u201dinitial\u201d background_position=\u201dtop_left\u201d background_repeat=\u201drepeat\u201d custom_padding=\u201d||0px||false|false\u201d hover_enabled=\u201d0\u2033 global_colors_info=\u201d{}\u201d sticky_enabled=\u201d0\u2033]<\/p>\n<h4 style=\"text-align: center;\"><strong>\ub9bc\ud504\ubaa8\uc138\ud3ec \ubc30\uc591 \ud504\ub85c\ud1a0\ucf5c<\/strong><\/h4>\n<h4><strong>\uc131\uc7a5 \ub9e4\uccb4<\/strong><\/h4>\n<p>RPMI 1640 \u2013 ThermoFisher # 11875<br \/>15% \ud0dc\uc544\uc6b0\ud608\uccad(FBS) \u2013 ThermoFisher #10437-028<br \/>1%(1X) \ud398\ub2c8\uc2e4\ub9b0-\uc2a4\ud2b8\ub819\ud1a0\ub9c8\uc774\uc2e0(\uc120\ud0dd \uc0ac\ud56d) \u2013 ThermoFisher #15140-122<\/p>\n<h4><strong><\/strong><\/h4>\n<h4><strong>\uc77c\ubc18 \ucc38\uace0 \uc0ac\ud56d<br \/><\/strong><\/h4>\n<ul>\n<li>\uc131\uc7a5 \ub9e4\uccb4\ub294 \ud56d\uc0c1 37\u00b0C, 5% CO\uc758 \uc778\ud050\ubca0\uc774\ud130\uc5d0\uc11c \ud3c9\ud615\uc744 \uc720\uc9c0\ud574\uc57c \ud569\ub2c8\ub2e4.<sub>2<\/sub> \uc0ac\uc6a9 \uc804 30\ubd84 \ub3d9\uc548<\/li>\n<li>\ud56d\uc0c1 \ud1b5\ud48d\uad6c\uac00 \uc788\ub294 \ucea1\uc774 \uc788\ub294 \ud50c\ub77c\uc2a4\ud06c\ub97c \uc0ac\uc6a9\ud558\uc138\uc694<\/li>\n<li>\ud56d\uc0c1 \ud50c\ub77c\uc2a4\ud06c\ub97c \uc218\uc9c1\uc73c\ub85c \uc138\uc6cc\uc11c \ubc30\uc591\ud558\uc2ed\uc2dc\uc624.<\/li>\n<li>T25 \ud50c\ub77c\uc2a4\ud06c\uc5d0\ub294 20ml \uc774\uc0c1\uc744 \uc0ac\uc6a9\ud574\uc11c\ub294 \uc548 \ub429\ub2c8\ub2e4.<\/li>\n<\/ul>\n<h4><strong><\/strong><\/h4>\n<h4><strong>\ub0c9\ub3d9 \uc138\ud3ec \ud574\ub3d9<br \/><\/strong><\/h4>\n<p>\ub3d9\uacb0\ub41c \uc138\ud3ec\ub294 \uac74\uc870 \uc5bc\uc74c \uc704\uc5d0 1ml \ubd84\ub7c9\uc73c\ub85c \uc81c\uacf5\ub429\ub2c8\ub2e4. \uc138\ud3ec \ubc30\uc591\uc744 \uc704\ud574 \ud574\ub3d9\ud560 \ub54c\uae4c\uc9c0 \uc138\ud3ec\ub97c \ub3d9\uacb0 \uc0c1\ud0dc\ub85c \uc720\uc9c0\ud558\uac70\ub098, \ubc14\ub85c \ubc30\uc591\uc744 \uc2dc\uc791\ud558\uc9c0 \uc54a\uc744 \uacbd\uc6b0 \uc561\uccb4 \uc9c8\uc18c \ubcf4\uad00\uc18c\uc5d0 \ubcf4\uad00\ud569\ub2c8\ub2e4. \uadf8\ub7ec\ub098 \ub098\uc911\uc5d0 \uc0ac\uc6a9\ud558\uae30 \uc704\ud574 \uc138\ud3ec\ub97c \ubcf4\uad00\ud558\ub824\ub294 \uacbd\uc6b0 \uc2e0\uc120\ud55c \uc2a4\ud1a1\uc744 \ubc30\uc591\ud558\uace0 \uc5ec\ub7ec \uc570\ud50c\uc744 \ub3d9\uacb0\ud558\ub294 \uac83\uc774 \uc88b\uc2b5\ub2c8\ub2e4. \uc138\ud3ec\ub294 45% RPMI-1640, 50% FBS \ubc0f 5% DMSO(\uc870\uc9c1 \ubc30\uc591 \ub4f1\uae09)\uc5d0\uc11c \ub0c9\ub3d9 \ubcf4\uad00\ud569\ub2c8\ub2e4.<\/p>\n<ol>\n<li style=\"list-style-type: none;\">\n<ol>\n<li>\ud1b5\ud48d\uad6c\uac00 \uc788\ub294 \ucea1\uc774 \ub2ec\ub9b0 T-25 \ud50c\ub77c\uc2a4\ud06c\uc5d0 RPMI-1640, 15% \ud0dc\uc544\uc6b0\uc2dc\ud608\uccad(FBS), \u00b1 \ud56d\uc0dd\uc81c 10ml\ub97c \ub123\uc2b5\ub2c8\ub2e4.<\/li>\n<li>5% CO\uac00 \ud3ec\ud568\ub41c 37\u00b0C \uc2b5\ub3c4 \uc778\ud050\ubca0\uc774\ud130\uc5d0\uc11c \ubc30\uc9c0\uac00 \ud3c9\ud615\uc744 \uc774\ub8e8\ub3c4\ub85d \ud569\ub2c8\ub2e4.<sub>2<\/sub> \uacf5\uae30 \uc911\uc5d0\uc11c 30\ubd84 \ub3d9\uc548. \ud50c\ub77c\uc2a4\ud06c\ub294 \uc218\uc9c1\uc73c\ub85c \ubc30\uc591\ud574\uc57c \ud569\ub2c8\ub2e4.<\/li>\n<li>\ub0c9\ub3d9\ub41c \uc138\ud3ec\uc758 \uc570\ud50c\uc774\ub098 \ubc14\uc774\uc54c\uc744 \ud55c \ubc88\uc5d0 \ud558\ub098\uc529 37\u00b0C \uc218\uc870\ub098 \ubbf8\uc9c0\uadfc\ud55c \ubb3c\uc774 \ub2f4\uae34 \ube44\uc774\ucee4\uc5d0 \ub123\uc5b4 \ud574\ub3d9\ud569\ub2c8\ub2e4.<\/li>\n<li>70% \uc5d0\ud0c4\uc62c\ub85c \uc570\ud50c\uc774\ub098 \ubc14\uc774\uc54c\uc758 \uc678\ubd80\ub97c \ube60\ub974\uac8c \ub2e6\uc2b5\ub2c8\ub2e4. \uc138\ud3ec\uac00 \uc720\ub9ac \uc570\ud50c\uc5d0 \ub4e4\uc5b4 \uc788\ub294 \uacbd\uc6b0, \uc190\uac00\ub77d\uc774 \uae68\uc9c4 \uc720\ub9ac\ub85c\ubd80\ud130 \ubcf4\ud638\ub418\ub3c4\ub85d \uc8fc\uc758\ud558\uba74\uc11c \uc570\ud50c\uc744 \uc870\uc2ec\uc2a4\ub7fd\uac8c \uae68\uc2ed\uc2dc\uc624.<\/li>\n<li>\uba78\uade0 \ud53c\ud3ab\uc73c\ub85c \ub3d9\uacb0\ub41c \uc138\ud3ec 1ml\ub97c \uaebc\ub0b4\uace0 10ml\uc758 \ubc30\uc9c0\uac00 \uc788\ub294 T-25 \ud50c\ub77c\uc2a4\ud06c\uc5d0 \ub123\uc2b5\ub2c8\ub2e4. \uac01 \uc138\ud3ec\uc8fc\ub97c \uc2dd\ubcc4\ud558\uae30 \uc704\ud574 \ud50c\ub77c\uc2a4\ud06c\uc5d0 \uba85\ud655\ud558\uac8c \ub77c\ubca8\uc744 \ubd99\uc785\ub2c8\ub2e4.<\/li>\n<li>\ud50c\ub77c\uc2a4\ud06c\ub97c 5% CO\uac00 \ub4e4\uc5b4 \uc788\ub294 37\u00b0C \uac00\uc2b5 \uc778\ud050\ubca0\uc774\ud130\uc5d0 \ub123\uc73c\uc138\uc694.<sub>2<\/sub> \uacf5\uae30 \uc911\uc5d0\uc11c. \ud50c\ub77c\uc2a4\ud06c\ub294 \uc218\uc9c1\uc73c\ub85c \ubc30\uc591\ud574\uc57c \ud569\ub2c8\ub2e4.<\/li>\n<li>24\uc2dc\uac04 \ud6c4, \ud50c\ub77c\uc2a4\ud06c \ubc14\ub2e5\uc5d0 \uac00\ub77c\uc549\uc740 \ub9bc\ud504\uad6c\ub97c \uac74\ub4dc\ub9ac\uc9c0 \uc54a\uace0 \uc704\ucabd\uc5d0\uc11c \ubc30\uc9c0 5ml\ub97c \uc81c\uac70\ud558\uace0, \ubbf8\ub9ac \ub370\uc6cc\ub454 \ubc30\uc9c0 5ml\ub85c \uad50\uccb4\ud569\ub2c8\ub2e4.<\/li>\n<li>\ub9bc\ud504\ubaa8\uc138\ud3ec \ubc30\uc591 \ud504\ub85c\ud1a0\ucf5c\uc744 \uc9c4\ud589\ud558\uc138\uc694.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<h4><strong><\/strong><\/h4>\n<h4><strong>\ub9bc\ud504\ubaa8\uc138\ud3ec \ubc30\uc591<\/strong><\/h4>\n<ol>\n<li style=\"list-style-type: none;\">\n<ol>\n<li>\ud574\ub3d9 \ud6c4 3~4\uc77c \ud6c4\uc5d0 \uc138\ud3ec\ub97c \uc138\uc2ed\uc2dc\uc624. \uc138\ud3ec\ub294 \ubd80\ucc29\ub418\uc9c0 \uc54a\uace0 \ub369\uc5b4\ub9ac\ub85c \uc790\ub78d\ub2c8\ub2e4. \ubd80\ub4dc\ub7fd\uac8c \ud53c\ud3ab\ud305\ud558\uc5ec \ub369\uc5b4\ub9ac\ub97c \ubd84\ud574\ud558\uc2ed\uc2dc\uc624.<\/li>\n<li>\uc138\ud3ec\ub97c \uc138\uc5b4\ubcf4\uc138\uc694.<\/li>\n<li>\uc774\uc0c1\uc801\uc73c\ub85c \uc138\ud3ec \ub18d\ub3c4\ub294 2 x 10\uc744 \ucd08\uacfc\ud574\uc11c\ub294 \uc548 \ub429\ub2c8\ub2e4.<sup>6<\/sup> \uc138\ud3ec\/ml.<\/li>\n<li>\uc138\ud3ec \ub18d\ub3c4\uc5d0 \ub530\ub77c \uc138\ud3ec\ub97c \uc7ac\uacf5\uae09, \ubd84\ud560 \ub610\ub294 \ub3d9\uacb0\ud569\ub2c8\ub2e4.<\/li>\n<li>\ud3c9\ud615\ud654\ub41c \uc131\uc7a5\ubc30\uc9c0 5~6ml\ub97c \ucd94\uac00\ud558\uc5ec \ub2e4\uc2dc \uacf5\uae09\ud569\ub2c8\ub2e4.<\/li>\n<li>\ucd5c\uc18c 2 x 10\uc758 \uc0c8\ub85c\uc6b4 \ubb38\ud654\uad8c\uc744 \uc2ec\uc73c\uc138\uc694<sup>5<\/sup> \uc138\ud3ec\/ml.<\/li>\n<li>\uc138\ud3ec\ub294 \uc57d 5 x 10\uc5d0\uc11c \ub3d9\uacb0\ub418\uc5b4\uc57c \ud569\ub2c8\ub2e4.<sup>6<\/sup> \uc138\ud3ec\/ml. \uc138\ud3ec \ub3d9\uacb0 \ud504\ub85c\ud1a0\ucf5c\uc744 \ub530\ub974\uc138\uc694.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<h4><strong><\/strong><\/h4>\n<h4><strong>\ub3d9\uacb0 \ub9bc\ud504\ubaa8\uc138\ud3ec<\/strong><\/h4>\n<ol>\n<li style=\"list-style-type: none;\">\n<ol>\n<li>\uc138\ud3ec\ub294 \uc57d 5 x 10\uc5d0\uc11c \ub3d9\uacb0\ub418\uc5b4\uc57c \ud569\ub2c8\ub2e4.<sup>6<\/sup> \uc138\ud3ec\/ml.<\/li>\n<li>\uc801\uc815\ub7c9\uc758 \uc138\ud3ec\ub97c 15 \ub610\ub294 50ml \uc6d0\ubfd4\ud615 \ud29c\ube0c\uc5d0 \uc62e\uae41\ub2c8\ub2e4.<\/li>\n<li>4\u00b0C\uc5d0\uc11c 100 xg\ub85c 10\ubd84\uac04 \uc6d0\uc2ec\ubd84\ub9ac\ud569\ub2c8\ub2e4.<\/li>\n<li>\ubc30\uc9c0\ub97c \uc81c\uac70\ud55c \ud6c4 \uc801\uc808\ud55c \uc591\uc758 \ucc28\uac00\uc6b4 \ub0c9\ub3d9 \ubc30\uc9c0\uc5d0 \ub2e4\uc2dc \ud604\ud0c1\uc2dc\ud0b5\ub2c8\ub2e4.<\/li>\n<li>\uac01 \uc138\ud3ec 1ml\ub97c \ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c\uc5d0 \uc62e\uae41\ub2c8\ub2e4.<\/li>\n<li>\ub0c9\ub3d9\uc2e4\uc5d0 \ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c\uc744 \ub123\uace0 -80\u00b0C \ub0c9\ub3d9\uace0\uc5d0 \ubc24\uc0c8 \ub123\uc5b4\ub461\ub2c8\ub2e4.<\/li>\n<li>\ub2e4\uc74c \ub0a0, \ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c\uc744 \uc561\uccb4\uc9c8\uc18c\ub85c \uc62e\uae41\ub2c8\ub2e4.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=\u201d4.27.4\u2033 _module_preset=\u201ddefault\u201d hover_enabled=\u201d0\u2033 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custom_padding=\u201d||0px|||\u201d custom_padding_tablet=\u201d|35px||35px||true\u201d custom_padding_phone=\u201d\u201d border_width_top=\u201d10px\u201d border_color_top=\u201d#8fd2ed\u201d global_colors_info=\u201d{}\u201d][et_pb_column_inner saved_specialty_column_type=\u201d3_4\u2033 _builder_version=\u201d4.16\u2033 custom_padding=\u201d|||\u201d global_colors_info=\u201d{}\u201d custom_padding__hover=\u201d|||\u201d][et_pb_text _builder_version=\u201d4.16\u2033 global_colors_info=\u201d{}\u201d]<\/p>\n<h4><strong>\uc790\uc138\ud55c \ub0b4\uc6a9\uc740 \ub2e4\uc74c \uc8fc\uc18c\ub85c \ubb38\uc758\ud558\uc138\uc694.<\/strong><\/h4>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner column_structure=&quot;1_2,1_2&quot; custom_padding_last_edited=&quot;on|phone&quot; _builder_version=&quot;4.16&quot; custom_padding=&quot;|35px|0|0px|false|false&quot; custom_padding_tablet=&quot;|35px||35px||true&quot; custom_padding_phone=&quot;&quot; animation_direction=&quot;top&quot; global_colors_info=&quot;{}&quot;][et_pb_column_inner type=&quot;1_2&quot; saved_specialty_column_type=&quot;3_4&quot; _builder_version=&quot;4.16&quot; custom_padding=&quot;|20px||&quot; custom_padding_tablet=&quot;||20px&quot; custom_padding_phone=&quot;&quot; custom_padding_last_edited=&quot;on|desktop&quot; global_colors_info=&quot;{}&quot; custom_padding__hover=&quot;|||&quot;][et_pb_text _builder_version=&quot;4.19.5&quot; global_colors_info=&quot;{}&quot;]<\/p>\n<h6>Leslie B. Gordon, MD, PhD<\/h6>\n<p>\ube0c\ub77c\uc6b4 \ub300\ud559\uad50\uc758 Warren Alpert \uc758\ub300 \uc18c\uc544\uacfc \uc5f0\uad6c \uad50\uc218, \ub85c\ub4dc\uc544\uc77c\ub79c\ub4dc \uc8fc \ud504\ub85c\ube44\ub358\uc2a4\uc5d0 \uc788\ub294 Hasbro \uc5b4\ub9b0\uc774 \ubcd1\uc6d0 \uc18c\uc544\uacfc, \ubcf4\uc2a4\ud134 \uc5b4\ub9b0\uc774 \ubcd1\uc6d0 \ub9c8\ucde8\uacfc, \ub9e4\uc0ac\ucd94\uc138\uce20 \uc8fc \ubcf4\uc2a4\ud134\uc5d0 \uc788\ub294 Harvard \uc758\ub300, Progeria Research Foundation \uc758\ub8cc \ucc45\uc784\uc790<\/p>\n<p>\uc804\ud654: 978-535-2594<br \/>\ud329\uc2a4: 508-543-0377<br \/><span> <\/span><a href=\"mailto:lgordon@progeriaresearch.org\" title=\"mailto:lgordon@progeriaresearch.org\">lgordon@progeriaresearch.org<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column_inner][et_pb_column_inner type=\u201d1_2\u2033 saved_specialty_column_type=\u201d3_4\u2033 _builder_version=\u201d4.16\u2033 custom_padding=\u201d|||20px\u201d custom_padding_tablet=\u201d20px|||0px\u201d custom_padding_phone=\u201d\u201d custom_padding_last_edited=\u201don|phone\u201d global_colors_info=\u201d{}\u201d custom_padding__hover=\u201d|||\u201d][et_pb_text _builder_version=\u201d4.27.2\u2033 custom_padding=\u201d||0px|||\u201d custom_padding_last_edited=\u201doff|desktop\u201d global_colors_info=\u201d{}\u201d]<\/p>\n<h6><span>\uc6ec\ub514 \ub178\ub9ac\uc2a4<\/span><\/h6>\n<div><span>PRF \uc138\ud3ec \ubc0f \uc870\uc9c1 \uc740\ud589<br \/>\uc804\ud654: 401-274-1122 x 48063<br \/><a href=\"mailto:wnorris@brownhealth.org\" title=\"mailto:wnorris@brownhealth.org\" data-outlook-id=\"6ae643af-3829-46f7-8b6a-2ef767034802\">wnorris@brownhealth.org<\/a><br \/><\/span><\/div>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=&quot;1&quot; 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Keep cells frozen until thawed for cell culture or place in liquid nitrogen storage if not starting cultures right away. However, it is recommended that if you are going to store the cells for later use, grow up fresh stocks and freeze multiple ampoules. The cells are cryopreserved in 45% RPMI-1640, 50% fetal calf serum, and 5% DMSO (tissue culture grade).\r\n<ol>\r\n \t<li>Place 10 ml of RPMI-1640, 15% fetal calf serum (FCS), \u00b1antibiotics in a T-25 flask.<\/li>\r\n \t<li>Allow the media to equilibrate in a 37\u00b0C humidified incubator with 5% CO 2in air for 30 minutes.<\/li>\r\n \t<li>Thaw each ampoule of frozen cells in a 37\u00b0C water bath or a beaker of lukewarm water, one at a time.<\/li>\r\n \t<li>Quickly clean the outside of the ampoule off with a sterile isopropanol prep pad, then wrap the prep pad around the ampoule to protect fingers and crack the seal on the ampoule inside a biological safety hood.<\/li>\r\n \t<li>Remove the 1 ml of frozen cells with a sterile glass Pasteur pipet and place in a T-25 flask with 10 ml of medium. Clearly label flask to identify each cell line.<\/li>\r\n \t<li>Place the cells in a 37\u00b0C humidified incubator with 5% CO 2in the air.<\/li>\r\n \t<li>After 24 hours remove 5 ml of medium from the top without disturbing lymphocytes settled on the bottom of the flask and replace with 5 ml of pre-warmed medium.<\/li>\r\n \t<li>Feed the cells with 5 - 6 ml of fresh medium every 3 - 4 days and split into new cultures as required.<\/li>\r\n<\/ol>\r\n<strong>From Shipment of Live Cultures<\/strong>\r\n<ol>\r\n \t<li>Live lymphocyte cultures will be received in T-25 flasks filled to the top with RPMI-1640, 15% FCS, 1% Penicillin-Streptomycin (Gibco). The caps will be tight and sealed with parafilm.<\/li>\r\n \t<li>Remove parafilm.<\/li>\r\n \t<li>Place cultures in a 37\u00b0C humidified incubator with 5% CO2 in air and allow cells to settle out (20 - 30 minutes).<\/li>\r\n \t<li>Using a sterile pipet remove all but 10 - 15 ml of medium and discard.<\/li>\r\n \t<li>Place caps back on flasks loosened slightly to allow gas exchange (Check to make sure cap threads do not have media on them. If so you may want to transfer to a new flask.) Place the cultures back in the incubator with 5% CO 2in air. They should recover in a day or two.<\/li>\r\n \t<li>Feed the cells with fresh medium every 3 - 4 days by resuspending the cell aggregates and removing 50 -60% of the volume and replacing with fresh media. Cells removed from the flask can be used to initiate new cultures as needed.<\/li>\r\n<\/ol>\r\n<strong>For Further Information Please Contact:<\/strong>\r\n\r\nLeslie B. Gordon, MD, PhD\r\nProfessor of Pediatrics Research\u00a0Warren Alpert Medical School of Brown University and Department of Pediatrics, Hasbro Children's Hospital, Providence, RI\u00a0Department of Anesthesia, Children's Hospital Boston and Harvard Medical School, Boston, MA\u00a0Medical Director, The Progeria Research Foundation\r\n\r\nPhone: 978-535-2594\r\nFax: 508-543-0377\r\n<a href=\"mailto:Leslie_Gordon@brown.edu\">Leslie_Gordon@brown.edu<\/a>\r\n<div><span style=\"font-family: Georgia, Palatino;\">Wendy Norris<\/span><\/div>\r\n<div><span style=\"font-family: Georgia, Palatino;\">PRF Cell and Tissue Bank\r\nPhone: 401-274-1122 x 48063\r\n<a href=\"mailto:wnorris@lifespan.org\" target=\"_blank\" rel=\"noopener noreferrer\">wnorris@lifespan.org<\/a><\/span><\/div>\r\n[\/vc_column_text][\/vc_column][\/vc_row]\t\t","_et_gb_content_width":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-1004","page","type-page","status-publish","hentry"],"yoast_head":"<!-- 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