{"id":1010,"date":"2017-02-27T21:26:38","date_gmt":"2017-02-27T21:26:38","guid":{"rendered":"https:\/\/www.progeriaresearch.org\/?page_id=1010"},"modified":"2026-03-17T10:50:58","modified_gmt":"2026-03-17T14:50:58","slug":"induced-pluripotent-stem-cells","status":"publish","type":"page","link":"https:\/\/www.progeriaresearch.org\/ko\/induced-pluripotent-stem-cells\/","title":{"rendered":"\uc720\ub3c4\ub9cc\ub2a5\uc904\uae30\uc138\ud3ec"},"content":{"rendered":"

[et_pb_section fb_built="1" \uc804\uccb4 \ub108\ube44="\ucf1c\uae30" \ube44\ud65c\uc131\ud654\ub428="\uaebc\uc9d0|\uaebc\uc9d0|\uaebc\uc9d0" _\ube4c\ub354_\ubc84\uc804="4.16" \ud14c\ub450\ub9ac_\ub108\ube44_\uc544\ub798="55px" \ud14c\ub450\ub9ac_\uc0c9\uc0c1_\uc544\ub798="#29327a" \uc7a0\uae40="\uaebc\uc9d0" \uae00\ub85c\ubc8c_\uc0c9\uc0c1_\uc815\ubcf4="{}"][et_pb_\uc804\uccb4 \ub108\ube44_\ud5e4\ub354 _\ube4c\ub354_\ubc84\uc804="4.16" \uc81c\ubaa9_\uae00\uaf34="||||||||" \uc81c\ubaa9_\uae00\uaf34_\ud06c\uae30="55" \ubc30\uacbd_\uc0c9\uc0c1="#29327a" \ubc30\uacbd_\uc774\ubbf8\uc9c0="https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/12\/Jes.jpg" \ubc30\uacbd_\uc704\uce58="center_left" \uc0ac\uc6a9\uc790 \uc9c0\uc815_\ud328\ub529="9vw|0px|9vw||true|" custom_padding_tablet="" custom_padding_phone="|56px||" custom_padding_last_edited="on|desktop" title_font_size_tablet="45px" title_font_size_phone="40px" title_font_size_last_edited="on|phone" z_index_tablet="500" custom_css_main_element="background-position: center 18% !important;" global_colors_info="{}"]<\/p>\n

\uc720\ub3c4 \ub2e4\ub2a5\uc131<\/h1>\n

\uc904\uae30\uc138\ud3ec<\/h1>\n

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[\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ use_custom_gutter=”on” gutter_width=”1″ specialty=”on” padding_left_1=”35px” padding_left_2=”35px” padding_2_tablet=”|||0px” padding_2_phone=”” padding_2_last_edited=”on|desktop” module_class_1=”sidebar-secondary-nav” module_class=”handprint-bg” _builder_version=”4.16″ background_image=”https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2019\/04\/blue-handprint-only.png” parallax=”on” parallax_method=”off” inner_width=”100%” inner_max_width=”100%” custom_padding=”0|0px|54px|0px|false|false” z_index_tablet=”500″ border_width_top=”10px” border_color_top=”#8fd2ed” use_custom_width=”on” width_unit=”off” custom_width_percent=”100%” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_sidebar area=”et_pb_widget_area_14″ disabled_on=”on|on|off” module_class=”subpage-sidebars” _builder_version=”4.16″ animation_style=”fade” z_index_tablet=”500″ border_width_right=”5px” locked=”off” global_colors_info=”{}”][\/et_pb_sidebar][\/et_pb_column][et_pb_column type=”3_4″ specialty_columns=”3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner custom_padding_last_edited=”on|phone” _builder_version=”4.27.5″ custom_padding=”|35px|0|0px|false|false” custom_padding_tablet=”|35px||35px||true” custom_padding_phone=”” animation_direction=”top” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”3_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.27.5″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_padding=”||0px||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

\ud504\ub85c\uac8c\ub9ac\uc544 \uc5f0\uad6c \uc7ac\ub2e8 \uc138\ud3ec \ubc0f \uc870\uc9c1 \uc740\ud589<\/strong> \uc778\uac04 \uc720\ub3c4 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec(iPSC)<\/h4>\n
    \n
  1. \ud504\ub85c\uac8c\ub9ac\uc544 iPSC <\/strong>\ube44\uacfc\ud559\uc790\ub97c \uc704\ud55c \ubc30\uacbd \uc815\ubcf4<\/strong><\/a>\u00a0<\/strong><\/li>\n
  2. \ubaa9\uc801 <\/strong>Progeria Research Foundation\uc758 \uc720\ub3c4 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec \uc0dd\uc131 \ubc0f \ubd84\ubc30\u00a0<\/strong>\u00a0<\/a><\/li>\n
  3. \ud5c8\uce5c\uc2a8-\uae38\ud3ec\ub4dc \ud504\ub85c\uac8c\ub9ac\uc544 \uc99d\ud6c4\uad70 \uc720\ub3c4 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec(iPSC) \uc0dd\uc131<\/strong>\u00a0<\/a><\/li>\n
  4. \ud488\uc9c8 \uad00\ub9ac: \uac80\uc99d \ubc0f \ud2b9\uc131\ud654<\/strong>\u00a0<\/a><\/li>\n
  5. iPSC\uac00 \uc720\ub798\ub41c \uc6d0\ub798 \uc2dc\uc791 \ubb3c\uc9c8<\/strong><\/a><\/li>\n
  6. \ud5a5\ud6c4 iPSC \uc5c5\ub370\uc774\ud2b8 \ubc0f \uc0c8\ub85c\uc6b4 \uc138\ud3ec\uc8fc\uc5d0 \ub300\ud55c \uc774\uba54\uc77c \ubaa9\ub85d\uc5d0 \uac00\uc785\ud558\uc138\uc694<\/strong>\u00a0\u00a0<\/strong><\/a><\/li>\n
  7. \uc9c8\ubb38\uc774 \uc788\uc73c\uc2e0\uac00\uc694? \uc800\ud76c\uc5d0\uac8c \uc5f0\ub77d\ud558\uc138\uc694.<\/strong><\/a><\/li>\n
  8. iPSC \ub77c\uc778 \uc8fc\ubb38<\/strong><\/a>\u00a0<\/strong><\/li>\n
  9. HGPS \ubc0f Control iPSC \ubc30\uc591 \ubc30\uc9c0 \uc900\ube44<\/strong>\u00a0<\/strong><\/a><\/li>\n
  10. \ub9c8\ud2b8\ub9ac\uc824 \ud50c\ub808\uc774\ud2b8 \uc900\ube44\u00a0<\/strong><\/a><\/li>\n
  11. hESC \ubc0f iPSC \uc138\ud3ec \ud574\ub3d9(\ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c \ub2f9)<\/strong><\/a><\/li>\n
  12. hESC\/iPSC \uc218\ud655 \ubc0f \uad00\ub9ac<\/strong>\u00a0<\/strong><\/a><\/li>\n
  13. \ud1b5\uacfc\ud558\ub2e4 <\/strong><\/a>hESC\/iPSC\u00a0<\/a><\/strong><\/li>\n
  14. hESC\/iPSC \ub3d9\uacb0<\/a><\/strong><\/li>\n<\/ol>\n

    [\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner custom_padding_last_edited=”on|phone” _builder_version=”4.27.5″ custom_padding=”42px|35px|0|0px|false|false” custom_padding_tablet=”|35px||35px||true” custom_padding_phone=”” animation_direction=”top” border_width_top=”10px” border_color_top=”#29327a” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”3_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text admin_label=”iPSC” module_id=”iPSC” _builder_version=”4.27.5″ custom_padding=”||0px||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

    1. \ube44\uacfc\ud559\uc790\ub97c \uc704\ud55c iPSC \ubc30\uacbd \uc815\ubcf4<\/strong><\/h4>\n

    \uc904\uae30\uc138\ud3ec\ub294 \uc544\uc9c1 \uc5b4\ub5a4 \uc138\ud3ec \uc720\ud615\uc774 \ub418\uc9c0 \uc54a\uc740 "\ubbf8\uc131\uc219" \uc138\ud3ec\uc785\ub2c8\ub2e4. \uc904\uae30\uc138\ud3ec\ub294 \uc2e0\uccb4\uc5d0\uc11c \uc2ec\uc7a5\uc774\ub098 \ud608\uad00\uc744 \uad6c\uc131\ud558\ub294 \uc138\ud3ec, \uae30\ud0c0 \uc870\uc9c1 \ubc0f \uc7a5\uae30\uc640 \uac19\uc774 \ub2e4\uc591\ud55c \uc720\ud615\uc758 \uc131\uc219 \uc138\ud3ec\ub85c \ubc1c\ub2ec\ud560 \uc7a0\uc7ac\ub825\uc774 \uc788\uae30 \ub54c\ubb38\uc5d0 \uc720\uc5f0\ud569\ub2c8\ub2e4. 2007\ub144 \uc5f0\uad6c\uc790\ub4e4\uc740 \uc5f0\uad6c \ubaa9\uc801\uc73c\ub85c \uc77c\ubc18\uc801\uc73c\ub85c \ubc30\uc591\ud558\ub294 \uc131\uc219\ud55c \uc131\uccb4 \uc138\ud3ec\ub97c \uc7ac\ud504\ub85c\uadf8\ub798\ubc0d\ud558\uc5ec \uc2e4\ud5d8\uc2e4\uc5d0\uc11c \uc904\uae30\uc138\ud3ec\ub97c \ub9cc\ub4dc\ub294 \uc804\ub7b5\uc744 \ubc1c\uacac\ud588\uc2b5\ub2c8\ub2e4.1, 2<\/sup><\/strong>\u00a0. \uc778\uacf5\uc801\uc73c\ub85c \uc0dd\uc131\ub41c \uc774\ub7ec\ud55c \uc904\uae30 \uc138\ud3ec\ub294 \uc720\ub3c4 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec("iPSC")\ub77c\uace0 \ud569\ub2c8\ub2e4. \ud504\ub85c\uac8c\ub9ac\uc544 \ubd84\uc57c\uc5d0\uc11c \uc774\ub294 \uc5c4\uccad\ub09c \ub3cc\ud30c\uad6c\uc785\ub2c8\ub2e4. \uacfc\ud559\uc790\ub4e4\uc740 \ucc98\uc74c\uc73c\ub85c \ud504\ub85c\uac8c\ub9ac\uc544 \uc904\uae30 \uc138\ud3ec\ub97c \ub9cc\ub4e4\uace0 \ud504\ub85c\uac8c\ub9ac\uc544\uc5d0\uc11c \uc904\uae30 \uc138\ud3ec\uac00 \uc5b4\ub5bb\uac8c \uae30\ub2a5\ud558\uace0 \ubc1c\ub2ec\ud558\ub294\uc9c0\uc5d0 \ub300\ud55c \uc9c8\ubb38\uc744 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc774\uc804\uc5d0\ub294 \uc778\uac04 \ud504\ub85c\uac8c\ub9ac\uc544 \uc904\uae30 \uc138\ud3ec\uc758 \uacf5\uae09\uc6d0\uc774 \uc5c6\uc5c8\uae30 \ub54c\ubb38\uc5d0 \ud504\ub85c\uac8c\ub9ac\uc544\uac00 \uc5c6\ub294 \uc0ac\ub78c\uc758 \uc904\uae30 \uc138\ud3ec\uc640 \ube44\uad50\ud558\uc5ec \ud504\ub85c\uac8c\ub9ac\uc544 \uc904\uae30 \uc138\ud3ec\uac00 \uc5b4\ub5bb\uac8c \uae30\ub2a5\ud558\ub294\uc9c0\uc5d0 \ub300\ud55c \uc815\ubcf4\uac00 \ubd80\uc871\ud588\uc2b5\ub2c8\ub2e4. \ub610\ud55c \uacfc\ud559\uc790\ub4e4\uc740 \ud504\ub85c\uac8c\ub9ac\uc544 \uc904\uae30 \uc138\ud3ec\ub97c \uc7ac\ud504\ub85c\uadf8\ub798\ubc0d\ud558\uc5ec \ucc98\uc74c\uc73c\ub85c \uc131\uc219\ud55c \ud504\ub85c\uac8c\ub9ac\uc544 \ud608\uad00, \uc2ec\uc7a5 \uc138\ud3ec \ubc0f \uae30\ud0c0 \uc138\ud3ec \uc720\ud615\uc744 \uc0dd\uc131\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc9c0\uae08\uae4c\uc9c0 \uc778\uac04 \ud504\ub85c\uac8c\ub9ac\uc544 \uc2ec\uc7a5 \ub610\ub294 \ud608\uad00 \uc138\ud3ec\uc758 \uacf5\uae09\uc6d0\uc774 \uc5c6\uc5c8\uc2b5\ub2c8\ub2e4.\u00a0\u00a0\uc774\uc81c \uc6b0\ub9ac\ub294 \ud0a4\ub97c \ubb3c\uc5b4\ubcfc \uc218 \uc788\uc2b5\ub2c8\ub2e4\u00a0<\/strong>\uc2ec\uc7a5\ub9c8\ube44\uc640 \ub1cc\uc878\uc911\uc73c\ub85c \uc778\ud574 \uc870\uae30 \uc0ac\ub9dd\ud558\ub294 \ud504\ub85c\uac8c\ub9ac\uc544\uc758 \uc2ec\uc7a5\ubcd1\uc5d0 \ub300\ud55c \uc9c8\ubb38. \uc6b0\ub9ac\ub294 \uc774\ub7ec\ud55c \ubc1c\uacac\uc744 \uc77c\ubc18 \uc778\uad6c\uc758 \uc2ec\uc7a5\ubcd1\uacfc \ub178\ud654\uc640 \ube44\uad50\ud558\uace0 \uc6b0\ub9ac \ubaa8\ub450\uc758 \ub178\ud654\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce58\ub294 \uc694\uc18c\uc5d0 \ub300\ud574 \ub354 \ub9ce\uc774 \uc54c\uc544\ub0bc \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ud504\ub85c\uac8c\ub9ac\uc544 \uc904\uae30 \uc138\ud3ec\ub97c \uc0ac\uc6a9\ud55c \ud6cc\ub96d\ud55c \uc5f0\uad6c\uac00 \uc774\ubbf8 \uc5ec\ub7ec \uac74 \ubc1c\ud45c\ub418\uc5c8\uc2b5\ub2c8\ub2e4.3-5<\/sup> \u00a0Progeria Research Foundation\uc758 \ubaa9\ud45c\ub294 \uc774 \uadc0\uc911\ud55c \ub3c4\uad6c\ub97c \uc0ac\uc6a9\ud558\uc5ec \ub354 \ub9ce\uc740 \ubc1c\uacac\uc744 \ucd09\uc9c4\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \uc904\uae30 \uc138\ud3ec\uc5d0 \ub300\ud55c \uc785\ubb38\uc11c\ub294 \uc774 \ubbf8\uad6d \uc815\ubd80 \uc6f9\uc0ac\uc774\ud2b8\ub97c \ucc38\uc870\ud558\uc138\uc694.\u00a0https:\/\/stemcells.nih.gov\/info\/basics.htm<\/a><\/p>\n

     <\/p>\n

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    1. \n
        \n
      1. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. \uc815\uc758\ub41c \uc694\uc778\uc5d0 \uc758\ud55c \uc131\uc778 \uc778\uac04 \uc12c\uc720\uc544\uc138\ud3ec\ub85c\ubd80\ud130 \ub2e4\ub2a5\uc131 \uc904\uae30\uc138\ud3ec \uc720\ub3c4.\u00a0\uc140.\u00a0<\/em>2007;131:861-872.<\/li>\n
      2. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin, II, Thomson JA. \uc778\uac04 \uccb4\uc138\ud3ec\ub85c\ubd80\ud130 \uc720\ub798\ub41c \uc720\ub3c4\ub9cc\ub2a5\uc904\uae30\uc138\ud3ec\uc8fc.\u00a0\uacfc\ud559.\u00a0<\/em>2007;318:1917-1920.<\/li>\n
      3. Liu GH, Barkho BZ, Ruiz S, Diep D, Qu J, Yang SL, Panopoulos AD, Suzuki K, Kurian L, Walsh C, Thompson J, Boue S, Fung HL, Sancho-Martinez I, Zhang K, Yates J, 3rd, Izpisua Belmonte JC. Hutchinson-Gilford progeria \uc99d\ud6c4\uad70\uc758 iPSC\ub97c \uc774\uc6a9\ud55c \uc870\uae30 \ub178\ud654\uc758 \uc7ac\ud604.\u00a0\uc790\uc5f0.\u00a0<\/em>2011;472:221-225.<\/li>\n
      4. \ubbf8\uc2a4\ud154\ub9ac T. HGPS \uc720\ub798 iPSC\ub294 \uc624\ub79c \uc138\uc6d4 \uc9c0\uc18d\ub418\uc5c8\uc2b5\ub2c8\ub2e4.\u00a0\uc138\ud3ec\uc904\uae30\uc138\ud3ec.\u00a0<\/em>2011;8:4-6.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/div>\n

        [\/et_pb_text][et_pb_text admin_label=”purpose” module_id=”purpose” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

        2. Progeria Research Foundation\uc758 \uc720\ub3c4\ub9cc\ub2a5\uc904\uae30\uc138\ud3ec(iPSC) \uc0dd\uc131 \ubc0f \ubc30\ud3ec \ubaa9\uc801<\/strong><\/h4>\n

        Progeria Research Foundation\uc758 \uc0ac\uba85\uc740 Hutchinson-Gilford Progeria \uc99d\ud6c4\uad70\uacfc \ub178\ud654 \uad00\ub828 \uc7a5\uc560\uc5d0 \ub300\ud55c \uce58\ub8cc\ubc95\uacfc \uc644\uce58\ubc95\uc744 \ubc1c\uacac\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. 2009\ub144 PRF\ub294 William Stanford \ubc15\uc0ac\uc758 \uc9c0\ud718 \uc544\ub798 \uce90\ub098\ub2e4 \ud1a0\ub860\ud1a0 \ub300\ud559\uc758 \uc804\ubb38\uac00 \uacfc\ud559\uc790 \ud300\uacfc \ud611\ub825\ud558\uc5ec \uace0\ud488\uc9c8 Progeria iPSC\ub97c \uc0dd\uc131\ud588\uc2b5\ub2c8\ub2e4. Stanford \ubc15\uc0ac\ub294 \ud1b5\ud569 \uc904\uae30 \uc138\ud3ec \uc0dd\ubb3c\ud559 \ubd84\uc57c\uc758 \uce90\ub098\ub2e4 \uc5f0\uad6c \uc758\uc7a5\uc785\ub2c8\ub2e4. 2011\ub144 \ud604\uc7ac PRF\ub294 \uce90\ub098\ub2e4 \uc624\ud0c0\uc640 \ub300\ud559\uc758 Stanford \ubc15\uc0ac\uc640 \ud611\ub825\uc744 \uacc4\uc18d\ud558\uace0 \uc788\uc73c\uba70, \uadf8\ub294 \uc758\ud559\ubd80 \uc138\ud3ec \ubc0f \ubd84\uc790 \uc758\ud559 \uad50\uc218\uc774\uc790 \uc624\ud0c0\uc640 \ubcd1\uc6d0 \uc5f0\uad6c\uc18c\uc758 Sprott \uc904\uae30 \uc138\ud3ec \uc5f0\uad6c \uc13c\ud130\uc758 \uc218\uc11d \uacfc\ud559\uc790\uc785\ub2c8\ub2e4.<\/p>\n

        \uc800\ud76c\uc758 \ubaa9\ud45c\ub294 \uc774 \uadc0\uc911\ud55c \ub3c4\uad6c\ub97c \uc804 \uc138\uacc4 \uc5f0\uad6c\uc790\ub4e4\uc5d0\uac8c \uc81c\uacf5\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \uc774 \uc0c8\ub85c\uc6b4 \uc5f0\uad6c \ub3c4\uad6c\ub294 \ud504\ub85c\uac8c\ub9ac\uc544\uc640 \uc2ec\uc7a5\ubcd1 \ubc0f \ub178\ud654\uc640\uc758 \uad00\uacc4\uc5d0 \ub300\ud55c \uc0c8\ub86d\uace0 \ud601\uc2e0\uc801\uc778 \uc5f0\uad6c\ub97c \uc0dd\uc131\ud558\ub294 \ub370 \uc0ac\uc6a9\ub420 \uac83\uc785\ub2c8\ub2e4.\u00a0\u00a0<\/strong><\/p>\n

        [\/et_pb_text][et_pb_text admin_label=”generation” module_id=”generation” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

        3. \ud5c8\uce5c\uc2a8-\uae38\ud3ec\ub4dc \ud504\ub85c\uac8c\ub9ac\uc544 \uc99d\ud6c4\uad70 \uc720\ub3c4 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec(iPSC) \uc0dd\uc131<\/strong><\/h4>\n

        \uc720\ub3c4\ub9cc\ub2a5\uc904\uae30\uc138\ud3ec(iPSC)\ub294 4\uac00\uc9c0 \uc778\uac04 \uc778\uc790\uc778 Oct4, Sox2, Klf4, c-Myc\ub97c \uc12c\uc720\uc544\uc138\ud3ec\ub85c VSVG-\uac00\uc131\ud615 \uc5ed\uc804\uc0ac\ubc14\uc774\ub7ec\uc2a4\ub85c \ud615\uc9c8 \ub3c4\uc785\ud558\uc5ec \uc720\ub798\ud588\uc2b5\ub2c8\ub2e4. iPSC \ucf5c\ub85c\ub2c8\ub294 \ub9c8\uc6b0\uc2a4 \ubc30\uc544 \uc12c\uc720\uc544\uc138\ud3ec(MEF)\uc5d0\uc11c \uc720\ub798\ud588\uc2b5\ub2c8\ub2e4. \uc0ac\uc6a9\ub41c \uc808\ucc28\ub294 \uae30\ubcf8\uc801\uc73c\ub85c \uc774\uc804\uc5d0 \uc124\uba85\ud55c \ub300\ub85c \ud588\uc9c0\ub9cc EOS \ub9ac\ud3ec\ud130\ub97c \uc0ac\uc6a9\ud558\uc9c0 \uc54a\uc558\uc2b5\ub2c8\ub2e4(Nature Protocols 4: 1828-1844, 2009).<\/p>\n

        [\/et_pb_text][et_pb_text admin_label=”quality” module_id=”quality” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ saved_tabs=”all” global_colors_info=”{}”]<\/p>\n

        4. \ud488\uc9c8 \uad00\ub9ac: \uac80\uc99d \ubc0f \ud2b9\uc131\ud654<\/strong><\/h4>\n

        \ud604\uc7ac \uc774\uc6a9 \uac00\ub2a5\ud55c \ub77c\uc778\uc740 \uc5ec\ub7ec \uac80\uc99d \ub2e8\uacc4\ub97c \uac70\ucce4\uc2b5\ub2c8\ub2e4(\uc544\ub798\uc5d0\uc11c \ub2e4\uc6b4\ub85c\ub4dc \uac00\ub2a5\ud55c PDF \ucc38\uc870):<\/p>\n

        \n
          \n
        1. \n
            \n
          1. \uac01 \uc138\ud3ec\uc8fc\uc5d0 \ub300\ud55c \ub9c8\uc774\ucf54\ud50c\ub77c\uc2a4\ub9c8 \uac80\uc0ac: \uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac\uc758 \uc5f0\uad6c\uc2e4\uc740 \uac01 \uc138\ud3ec\uc8fc\uc5d0 \ub300\ud574 PCR\uc744 \ud1b5\ud55c \ub9c8\uc774\ucf54\ud50c\ub77c\uc2a4\ub9c8 \ubd84\uc11d\uc744 \uc218\ud589\ud588\uc2b5\ub2c8\ub2e4. \ub610\ud55c, \uc138\ud3ec\ub97c \ud655\uc7a5\ud558\uace0 \ubc30\uc1a1\ud558\uae30 \uc804\uc5d0 \uc138\ud3ec\uc8fc\uc5d0 \ub300\ud574 \ub9c8\uc774\ucf54\ud50c\ub77c\uc2a4\ub9c8 \uac80\uc0ac\ub97c \ub2e4\uc2dc \uc2e4\uc2dc\ud569\ub2c8\ub2e4.<\/li>\n
          2. \ub2e4\ub2a5\uc131 \ub9c8\ucee4 Tra-1-60, Tra-1-81 \ubc0f SSEA4\uc5d0 \ub300\ud55c \uba74\uc5ed\uc5fc\uc0c9.<\/li>\n
          3. \ub2e4\ub2a5\uc131 \uc9c0\ud45c\ub85c\uc11c\uc758 \uc54c\uce7c\ub9ac\uc131 \uc778\uc0b0\uac00\uc218\ubd84\ud574\ud6a8\uc18c \uc5fc\uc0c9<\/li>\n
          4. \ubc30\uc544\uccb4 \ud615\uc131 \ubc0f \uc138 \ubc30\uc5fd \ub9c8\ucee4\uc5d0 \ub300\ud55c \ud6c4\uc18d \uba74\uc5ed \uc5fc\uc0c9. \ud14c\uc2a4\ud2b8\ub41c \ub9c8\ucee4\ub294 \u03b2III-\ud29c\ubd88\ub9b0(\uc678\ubc30\uc5fd), \ud3c9\ud65c\uadfc \uc561\ud2f4(\uc911\ubc30\uc5fd), Gata4 \ub610\ub294 AFP(\ub0b4\ubc30\uc5fd)\uc785\ub2c8\ub2e4.<\/li>\n
          5. \ud575\ud615 \ubd84\uc11d.<\/li>\n
          6. \ubd84\ud654\uc138\ud3ec\uc5d0\uc11c\uc758 \ub77c\ubbfc A\uc758 \uc7ac\ubc1c\ud604<\/li>\n
          7. \ud14c\ub77c\ud1a0\ub9c8 \uac80\uc0ac<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n

            \ucd94\uac00 \uac80\uc99d \uc9c4\ud589 \uc911:<\/strong>
            \uc77c\ubd80 \ub77c\uc778\uc740 \uc9c0\uc6d0 \ub370\uc774\ud130\uc5d0 \ud45c\uc2dc\ub41c \ub300\ub85c \ud14c\ub77c\ud1a0\ub9c8 \uac80\uc815\uc744 \uc644\ub8cc\ud588\uc2b5\ub2c8\ub2e4. \ub2e4\ub978 \ubaa8\ub4e0 \ub77c\uc778\uc758 \uacbd\uc6b0 \ud14c\ub77c\ud1a0\ub9c8 \uac80\uc815\uc774 \uc9c4\ud589 \uc911\uc774\uba70 \uc774\ub7ec\ud55c \uac80\uc815\uc774 \uc644\ub8cc\ub418\uba74 \uc0c1\ud0dc\uac00 \uc5c5\ub370\uc774\ud2b8\ub429\ub2c8\ub2e4.<\/p>\n<\/div>\n

            [\/et_pb_text][et_pb_text admin_label=”original” module_id=”original” _builder_version=”4.27.5″ custom_padding=”||0px|||” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

            5. \uc774\ub4e4 iPS \uc138\ud3ec\uac00 \uc720\ub798\ub41c \uc6d0\ub798 \uc6d0\ub8cc \ubb3c\uc9c8<\/strong><\/h4>\n

            iPSC\ub294 PRF \uc138\ud3ec \ubc0f \uc870\uc9c1 \uc740\ud589\uc758 \ud615\uc9c8\uc804\ud658\ub418\uc9c0 \uc54a\uc740 \uc12c\uc720\uc544\uc138\ud3ec \uc138\ud3ec\uc8fc\uc5d0\uc11c \uc720\ub798\ub418\uc5c8\uc2b5\ub2c8\ub2e4.<\/p>\n

            \ubaa8\ub4e0 iPS \uc138\ud3ec\uc8fc\uc5d0 \uc0ac\uc6a9\ub41c \ud615\uc9c8\ub3c4\uc785 \ubc29\ubc95\uc740 \ub808\ud2b8\ub85c\ubc14\uc774\ub7ec\uc2a4 MKOS\uc600\uc2b5\ub2c8\ub2e4.<\/p>\n

            <\/p>\n

            [\/et_pb_text][et_pb_text admin_label=”Table” module_id=”original” _builder_version=”4.27.5″ custom_padding=”||0px|||” hover_enabled=”0″ z_index_tablet=”500″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

            \n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
            iPSC \ub77c\uc778 ID<\/b><\/th>\ub3cc\uc5f0\ubcc0\uc774<\/b><\/th>\uc131\ubcc4 \ubc0f \uae30\ubd80 \uc5f0\ub839<\/b><\/th>\uae30\uc6d0 \uc138\ud3ec \uc720\ud615 \uc5ec\uae30\ub97c \ud074\ub9ad\ud558\uc138\uc694<\/a>.<\/b><\/th>\uc9c0\uc6d0 \ub370\uc774\ud130<\/b><\/th>\n<\/tr>\n<\/thead>\n
            HGADFN003 iPS 1B<\/td> LMNA\uc5d1\uc190 11,
            \n1824 C>T<\/td>            		\ub0a8\uc790 2\ub144 0\uac1c\uc6d4<\/td> \ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec
            \nHGADFN003 (\uc8fc)<\/td>            		003 \uc544\uc774\ud53c\uc5d0\uc2a41B<\/a><\/td>\n<\/tr>\n
            HGADFN003 iPS 1C<\/td> LMNA \uc5d1\uc190 11,
            \n1824 C>T<\/td>            		\ub0a8\uc790 2\ub144 0\uac1c\uc6d4<\/td> \ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec
            \nHGADFN003 (\uc8fc)<\/td>            		003 \uc544\uc774\ud53c\uc5d0\uc2a41C<\/a><\/td>\n<\/tr>\n
            HGDFN003
            \niPS 1D<\/td>            		LMNA \uc5d1\uc190 11,
            \n1824 C>T<\/td>            		\ub0a8\uc790 2\ub144 0\uac1c\uc6d4<\/td> \ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec
            \nHGADFN003 (\uc8fc)<\/td>            		003 iPS1D<\/a><\/td>\n<\/tr>\n
            HGADFN167 iPS 1J<\/td> LMNA \uc5d1\uc190 11, 1824 C>T<\/td>\ub0a8\uc790 8\uc138 5\uac1c\uc6d4<\/td>\ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec HGADFN167<\/td>167\ub9c8\ub825 1\uc8fc<\/a><\/td>\n<\/tr>\n
            HGADFN167 iPS 1Q<\/td> LMNA \uc5d1\uc190 11, 1824 C>T<\/td>\ub0a8\uc790 8\uc138 5\uac1c\uc6d4<\/td>\ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec HGADFN167<\/td>167 \uc544\uc774\uc5d0\uc2a4\ud53c\uc5d0\uc2a41\ud050<\/a><\/td>\n<\/tr>\n
            HGMDFN090 iPS 1B<\/td> HGADFN167\uc758 \uc5b4\uba38\ub2c8(\uc601\ud5a5\ubc1b\uc9c0 \uc54a\uc74c)<\/td>\uc5ec\uc790 37\uc138 10\uac1c\uc6d4<\/td>\ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec
            \nHGMDFN090<\/td>            		090 \uc544\uc774\ud53c\uc5d0\uc2a41B<\/a><\/td>\n<\/tr>\n
            HGMDFN090 iPS 1C<\/td> HGADFN167\uc758 \uc5b4\uba38\ub2c8(\uc601\ud5a5\ubc1b\uc9c0 \uc54a\uc74c)<\/td>\uc5ec\uc790 37\uc138 10\uac1c\uc6d4<\/td>\ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec
            \nHGMDFN090<\/td>            		090 iPS1C<\/a><\/td>\n<\/tr>\n
            HGFFDN168 iPS1 D2<\/td>HGADFN167\uc758 \uc544\ubc84\uc9c0(\uc601\ud5a5\ubc1b\uc9c0 \uc54a\uc74c)<\/td>\ub0a8\uc790 40\uc138
            \n5\uac1c\uc6d4<\/td>            		\ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec HGFFDN168<\/td>168 iPS1 D2<\/a><\/td>\n<\/tr>\n
            HGFFDN168 iPS1P<\/td>HGADFN167\uc758 \uc544\ubc84\uc9c0(\uc601\ud5a5\ubc1b\uc9c0 \uc54a\uc74c)<\/td>\ub0a8\uc790 40\uc138
            \n5\uac1c\uc6d4<\/td>            		\ud53c\ubd80\uc12c\uc720\uc544\uc138\ud3ec
            \nHGFFDN168<\/td>            		168 \uc544\uc774\uc5d0\uc2a4\ud53c\uc5d0\uc2a41P<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/h4>\n

            [\/et_pb_text][et_pb_button button_url=”https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2023\/05\/PRF-AVAILABLE-CELL-LINES-5-24-23.pdf” button_text=”PRF Available Cell Lines” button_alignment=”left” admin_label=”Cell lines pdf” _builder_version=”4.27.5″ background_layout=”dark” custom_margin=”||25px||false|false” hover_enabled=”0″ z_index_tablet=”500″ global_colors_info=”{}” sticky_enabled=”0″][\/et_pb_button][et_pb_text admin_label=”join” module_id=”join” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

            6. \ud5a5\ud6c4 iPSC \uc5c5\ub370\uc774\ud2b8 \ubc0f \uc0c8\ub85c\uc6b4 \uc138\ud3ec\uc8fc\uc5d0 \ub300\ud55c \uc774\uba54\uc77c \ubaa9\ub85d\uc5d0 \uac00\uc785\ud558\uc138\uc694.<\/strong><\/h4>\n

            \uc6b0\ub9ac\ub294 iPSC \ub77c\uc778\uc744 \uacc4\uc18d \uc0dd\uc131\ud558\uace0 \uc788\uc2b5\ub2c8\ub2e4. PRF \uc138\ud3ec \ubc0f \uc870\uc9c1 \uc740\ud589\uc5d0 \ubcf4\uad00\ub41c iPSC\uc5d0 \ub300\ud55c \uc8fc\uae30\uc801 \uc5c5\ub370\uc774\ud2b8\ub97c \uc6d0\ud558\uc2dc\uba74 \ud074\ub9ad\ud558\uc5ec \uc774\uba54\uc77c \ubaa9\ub85d\uc5d0 \uac00\uc785\ud558\uc138\uc694.\u00a0\uc5ec\uae30<\/a><\/strong><\/p>\n

            [\/et_pb_text][et_pb_text admin_label=”questions” module_id=”questions” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

            7. \uc9c8\ubb38\uc788\uc73c\uc138\uc694?<\/strong><\/h4>\n

            <\/a>\uc9c8\ubb38\uc774\ub098 \ud544\uc694 \uc0ac\ud56d\uc774 \uc788\uc73c\uc2dc\uba74 \uc758\ub8cc \ucc45\uc784\uc790\uc778 Leslie Gordon, MD, PhD\uc5d0\uac8c \uc5f0\ub77d\ud574 \uc8fc\uc2ed\uc2dc\uc624. lgordon@progeriaresearch.org<\/a>\u00a0\ub610\ub294 978-535-2594<\/p>\n

            [\/et_pb_text][et_pb_text admin_label=”ordering” module_id=”ordering” _builder_version=”4.27.5″ custom_padding=”||0px||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

            8. iPS \uc138\ud3ec\uc8fc \uc8fc\ubb38<\/strong><\/h4>\n

            2014\ub144\uc5d0 PRF\ub294 MTA\uc5d0 \ub300\ud55c \ubcc0\uacbd \uc5c6\uc74c \uc815\ucc45\uc744 \uc81c\uc815\ud588\uc2b5\ub2c8\ub2e4. \uc774\ub294 14\uac1c\uad6d\uc758 \uae30\uad00\uc5d0\uc11c \uc77c\ud558\ub294 70\uac1c \uc5f0\uad6c\ud300\uacfc 12\ub144\uac04\uc758 \uacc4\uc57d\uc801 \ud569\uc758\uc758 \uacb0\uacfc\uc785\ub2c8\ub2e4. PRF\uc640 \uadf8 \uc790\ubb38\uc740 \uadf8 \uae30\uac04 \ub3d9\uc548 \ubc1c\uc0dd\ud55c \ubb38\uc81c\ub97c \uace0\ub824\ud558\uace0 \uadf8\uc5d0 \ub530\ub77c \uacc4\uc57d\uc744 \ud3b8\uc9d1\ud558\uc5ec \uc6b0\ub9ac\uac00 \uacf5\uc815\ud558\uace0 \ud569\ub9ac\uc801\uc778 \uc870\uac74\uc774\ub77c\uace0 \uc0dd\uac01\ud558\ub294 \uacb0\uacfc\ub97c \uc5bb\uc5c8\uc2b5\ub2c8\ub2e4.<\/p>\n

            \ubbf8\uad6d \uc5f0\ubc29 \uc815\ubd80 \uae30\uad00 \ub610\ub294 \ubb38\uc758\uc0ac\ud56d\uc740 Wendy Norris\uc5d0\uac8c \ubb38\uc758\ud558\uc138\uc694. wnorris@brownhealth.org<\/a><\/a>\u00a0\ub610\ub294\u00a0401-274-1122 x 48063<\/a>.<\/p>\n

            1\ub2e8\uacc4: \uc2e0\uccad\uc11c \ubc0f \ubb3c\uc9c8 \uc774\uc804 \uacc4\uc57d\uc11c \uc791\uc131
            <\/strong>
            <\/a>            \ube44\uc815\ubd80\uae30\uad00 \uc2e0\uccad \ubc0f \ud611\uc815<\/a><\/p>\n

            \ube44\uc815\ubd80 \uae30\uad00\uc744 \uc704\ud55c \ubb3c\uc9c8 \uc774\uc804 \uacc4\uc57d<\/a><\/p>\n

            2\ub2e8\uacc4: \uc644\uc131\ub41c \uc2e0\uccad\uc11c\uc640 \uc790\uc7ac \uc774\uc804 \uacc4\uc57d\uc11c\ub97c Wendy Norris\uc5d0\uac8c \ubc18\ud658\ud558\uc2ed\uc2dc\uc624. wnorris@brownhealth.org<\/a>\uc2b9\uc778\ub418\uba74 \uc8fc\ubb38 \ud655\uc778 \ubc0f \uc608\uc0c1 \ubc30\uc1a1 \ub0a0\uc9dc\uac00 \uc801\ud78c \uc774\uba54\uc77c\uc744 \ubc1b\uac8c \ub429\ub2c8\ub2e4.<\/b>\u00a0<\/strong><\/a><\/p>\n

            3\ub2e8\uacc4: <\/strong>\uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac\uc758 \uc5f0\uad6c\uc2e4\uc740 \ud604\uc7ac \ub0c9\ub3d9 \ud06c\ub77c\uc774\uc624\ubc14\uc774\uc5bc\uc5d0 \ub77c\uc778\uc744 \ubc30\ud3ec\ud558\uace0 \uc788\uc2b5\ub2c8\ub2e4. \uadf8\uc758 \uc5f0\uad6c\uc2e4\uc740 \ubc30\uc591\ubb3c\uc774 \ubc1c\uc1a1\ub418\uba74 \ubc1c\uc1a1 \ubc0f \ucd94\uc801 \uc815\ubcf4\uc640 \ud568\uaed8 \uc774\uba54\uc77c\uc744 \ubcf4\ub0b4\ub4dc\ub9bd\ub2c8\ub2e4. \uacbd\ud5d8\uc774 \ubd80\uc871\ud55c \uc5f0\uad6c\uc790\ub294 \uc778\uac04 \ubc30\uc544\uc904\uae30\uc138\ud3ec\/iPSC \uc791\uc5c5\uc5d0 \ud544\uc218\uc801\uc778 \uc804\ubb38 \uacfc\uc815\uc5d0\uc11c \uad50\uc721\uc744 \ubc1b\ub3c4\ub85d \uc9c0\uc2dc\ubc1b\uc2b5\ub2c8\ub2e4.<\/p>\n

            \uc624\ud0c0\uc640 \ubcd1\uc6d0 \uc5f0\uad6c\uc18c\uc758 \uc778\uac04 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec \uc2dc\uc124(\uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac\uac00 \uc9c0\ud718)\uc740 Progeria iPSC \uc138\ud3ec\uc8fc\uc5d0 \ud2b9\ud654\ub41c iPSC \ubc30\uc591 \uae30\uc220\uc758 \uae30\ubcf8\uc5d0 \ub300\ud55c \uac00\uc0c1 \uc77c\ub300\uc77c \uad50\uc721\uc744 \uc81c\uacf5\ud569\ub2c8\ub2e4. \uad50\uc721 \uc635\uc158\uacfc \ud615\uc2dd\uc740 \uacfc\ud559\uc790\uc758 \uacbd\ud5d8 \uc218\uc900\uc5d0 \ub530\ub77c \uc720\uc5f0\ud558\uac8c \ub2ec\ub77c\uc9d1\ub2c8\ub2e4. \u00a0\uc790\uc138\ud55c \ub0b4\uc6a9\uc740 hpscf@ohri.ca\ub85c \uc774\uba54\uc77c\uc744 \ubcf4\ub0b4\uc8fc\uc138\uc694.<\/a>.<\/u><\/strong><\/p>\n

            4\ub2e8\uacc4: \uc624\ud0c0\uc640 \ub300\ud559\uad50\uc5d0\uc11c\ub294 \uac01 iPSC \ub77c\uc778\uc5d0 \ub300\ud574 \uc9c1\uc811 \uccad\uad6c\uc11c\ub97c \ubc1c\uc1a1\ud558\uba70, \ud0dd\ubc30 \ube44\uc6a9\ub3c4 \ud568\uaed8 \uccad\uad6c\ud569\ub2c8\ub2e4(\uc788\ub294 \uacbd\uc6b0).<\/strong><\/p>\n

            [\/et_pb_text][et_pb_text admin_label=”mediaprep” module_id=”mediaprep” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

            9. HGPS \ubc0f Control iPS \uc138\ud3ec \ubc30\uc591 \ubc30\uc9c0 \uc900\ube44<\/strong><\/h4>\n

            iPSC\uc640 ESC\ub294 Stem Cell Technologies(cat# 5825)\uc758 mTeSR Plus\ub97c \uacf5\uae09\ud574\uc57c \ud569\ub2c8\ub2e4. \ubcf4\uad00\uc5d0 \ub300\ud55c \uacf5\uae09\uc5c5\uccb4\uc758 \uad8c\uc7a5 \uc0ac\ud56d\uc744 \ub530\ub974\uc138\uc694.<\/p>\n

            [\/et_pb_text][et_pb_text admin_label=”surfaces” module_id=”surfaces” _builder_version=”4.27.5″ custom_padding=”||0px||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

            10. \ub9c8\ud2b8\ub9ac\uc824 \ud50c\ub808\uc774\ud2b8 \uc900\ube44<\/strong><\/h4>\n

            \uba54\ubaa8<\/strong>: \ub9c8\ud2b8\ub9ac\uc824\uacfc \uad00\ub828\ub41c \ubaa8\ub4e0 \ub2e8\uacc4\ub294 \uac00\ub2a5\ud55c \ud55c \ube68\ub9ac \uc774\ub8e8\uc5b4\uc838\uc57c \ud558\uba70 \uac00\ub2a5\ud55c \ud55c \ucc28\uac11\uac8c \uc720\uc9c0\ud574\uc57c \ud569\ub2c8\ub2e4.<\/p>\n

              \n
            1. \ub9c8\ud2b8\ub9ac\uc824 \ubcd1\uc744 4\ub3c4\uc5d0\uc11c \ub179\uc785\ub2c8\ub2e4.\u00b0<\/strong>C. \ud574\ub2f9 \ud488\ubaa9\uc758 \ubd84\uc11d \uc778\uc99d\uc11c\ub97c \ud655\uc778\ud558\uc5ec \ub2e8\ubc31\uc9c8 \ub18d\ub3c4\ub97c \ud655\uc778\ud558\uc138\uc694.<\/li>\n
            2. \ud574\ub3d9\ub41c \ub9c8\ud2b8\ub9ac\uc824\uc5d0 \ucd5c\uc885 \ub18d\ub3c4\uac00 5mg\/mL\uc774 \ub420 \ub54c\uae4c\uc9c0 \ucc28\uac00\uc6b4 DMEM\/F12 \ubc30\uc9c0\ub97c \ucda9\ubd84\ud788 \ucca8\uac00\ud569\ub2c8\ub2e4.<\/li>\n
            3. 2\ub2e8\uacc4\uc5d0\uc11c \uc900\ube44\ud55c \ub9c8\ud2b8\ub9ac\uc824\uc744 \ubbf8\ub9ac \ub0c9\uac01\ud574 \ub454 15mL \ud314\ucf58 \ud29c\ube0c\uc5d0 1mL\uc529 \ubd84\uc8fc\ud569\ub2c8\ub2e4.<\/li>\n
            4. \ubaa8\ub4e0 \uc54c\ub9ac\ucffc\ud2b8\ub97c -20\uc5d0\uc11c \ub3d9\uacb0\ud558\uace0 \ubcf4\uad00\ud558\uc2ed\uc2dc\uc624.\u00b0<\/strong>\uae30\uc74c.<\/li>\n
            5. \ub9c8\ud2b8\ub9ac\uac94 \ud50c\ub808\uc774\ud2b8\ub97c \ub9cc\ub4e4\ub824\uba74 -20\uc5d0\uc11c \ub9c8\ud2b8\ub9ac\uac94 \ud55c \uc870\uac01(1mL)\uc744 \uaebc\ub0c5\ub2c8\ub2e4.\u00b0<\/strong>C\uc5d0 \ucc28\uac00\uc6b4 DMEM\/F12 10mL\ub97c \ub354\ud569\ub2c8\ub2e4. \ud3a0\ub9bf\uc774 \ub179\uc744 \ub54c\uae4c\uc9c0 \uc798 \uc11e\uc2b5\ub2c8\ub2e4(\uac70\ud488\uc774 \uc0dd\uae30\uc9c0 \uc54a\uac8c \ud558\uace0, \uc6a9\uc561\uc740 \ud56d\uc0c1 \ucc28\uac11\uac8c \uc720\uc9c0).<\/li>\n
            6. 50mL \ud29c\ube0c\uc5d0 \uc62e\uae34 \ud6c4, \ucc28\uac00\uc6b4 DMEM\/F12 20mL\uc744 \ucca8\uac00\ud569\ub2c8\ub2e4(\ub9c8\ud2b8\ub9ac\uac94 \ubd84\uc8fc\ubb3c 1mL\uc744 DMEM\/F12 30mL\uc5d0 \ud76c\uc11d). \uc798 \uc11e\uc2b5\ub2c8\ub2e4.<\/li>\n
            7. \ud50c\ub808\uc774\ud2b8(6\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uacbd\uc6b0 1mL\/\uc6f0, 12\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uacbd\uc6b0 0.5mL\/\uc6f0, 24\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uacbd\uc6b0 0.25mL\/\uc6f0). \ud50c\ub808\uc774\ud2b8\ub97c \uac00\ubccd\uac8c \ud754\ub4e4\uc5b4 \uc6a9\uc561\uc774 \uc804\uccb4 \ud45c\uba74\uc744 \ub36e\uace0 \uc788\ub294\uc9c0 \ud655\uc778\ud558\uc2ed\uc2dc\uc624. \ub0a8\uc740 \ub9c8\ud2b8\ub9ac\uc824\uc744 \ub2e4\uc2dc \uc5bc\ub9ac\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.<\/li>\n
            8. \ud50c\ub808\uc774\ud2b8\ub97c \uc989\uc2dc \uc0ac\uc6a9\ud558\ub294 \uacbd\uc6b0 \ud50c\ub808\uc774\ud2b8\ub97c \uc2e4\uc628\uc5d0 1\uc2dc\uac04(\ub610\ub294 37\ub3c4\uc5d0\uc11c 30\ubd84) \ubc29\uce58\ud569\ub2c8\ub2e4.\u00b0<\/strong>C), \ud604\ubbf8\uacbd\uc73c\ub85c \ub9c8\ud2b8\ub9ac\uc824\uc744 \uad00\ucc30\ud569\ub2c8\ub2e4. \ub9c8\ud2b8\ub9ac\uc824\uc740 \uc798 \ubd84\uc0b0\ub418\uc5b4\uc57c \ud558\uba70 "\ubb49\uccd0\uc9c0\uc9c0" \uc54a\uc544\uc57c \ud569\ub2c8\ub2e4.<\/li>\n
            9. \ub2e4\ub978 \uc2dc\uac04\uc5d0 \ud50c\ub808\uc774\ud2b8\ub97c \uc0ac\uc6a9\ud558\ub294 \uacbd\uc6b0 \ud50c\ub808\uc774\ud2b8 \uac00\uc7a5\uc790\ub9ac\ub97c \ud30c\ub77c\ud544\ub984\uc73c\ub85c \uac10\uc2f8\uace0 4\uc5d0\uc11c \ubcf4\uad00\ud558\uc2ed\uc2dc\uc624.\u00b0<\/strong>C \ucd5c\ub300 2\uc8fc \ub3d9\uc548.<\/li>\n<\/ol>\n

              \ub9c8\ud2b8\ub9ac\uac94 \u2013 BD\/\ud53c\uc154, cat#CB-40230<\/p>\n

              DMEM\/F12 \u2013 Life Technologies, \uce74\ud0c8\ub85c\uadf8 #11330-057<\/p>\n

              \uc70c\ub9ac\uc5c4 \uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac-2022<\/p>\n

              [\/et_pb_text][et_pb_text admin_label=”inactivating” module_id=”inactivating” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

              11. <\/strong>ES \ub610\ub294 iPS \uc138\ud3ec \ud574\ub3d9(\ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c \ub2f9)<\/strong><\/h4>\n
                \n
              1. 4\u00b0C\uc5d0\uc11c \ub9c8\ud2b8\ub9ac\uac94 \ud50c\ub808\uc774\ud2b8\ub97c \uaebc\ub0b4\uc5b4 \uc2e4\uc628\uc5d0\uc11c 1\uc2dc\uac04 \ub3d9\uc548 \ub370\uc6b0\uac70\ub098, \uc0c8\ub85c\uc6b4 \ub9c8\ud2b8\ub9ac\uac94 \ud50c\ub808\uc774\ud2b8\ub97c \ub9cc\ub4ed\ub2c8\ub2e4(\ub9c8\ud2b8\ub9ac\uac94 \ud50c\ub808\uc774\ud2b8 \uc900\ube44 \ud504\ub85c\ud1a0\ucf5c \ucc38\uc870).<\/li>\n
              2. 15mL \ud314\ucf58 \ud29c\ube0c\uc5d0 mTeSR Plus 4mL\ub97c \ub123\uace0 \ub530\ub73b\ud558\uac8c \ub370\uc6c1\ub2c8\ub2e4.<\/li>\n
              3. \uc561\uccb4\uc9c8\uc18c \ud0f1\ud06c\uc5d0\uc11c \uc138\ud3ec\ub97c \uaebc\ub0b4\uace0 37\u00b0C \uc695\uc870\uc5d0\uc11c \uc791\uc740 \uc5bc\uc74c \uc870\uac01\ub9cc \ub0a8\uc744 \ub54c\uae4c\uc9c0 \ub3cc\ub9bd\ub2c8\ub2e4. \ubc14\uc774\uc54c\uc740 1-2\ubd84 \uc548\uc5d0 \ud574\ub3d9\ub429\ub2c8\ub2e4. \uc774 \ub2e8\uacc4\ub294 \ube68\ub9ac \ud574\uc57c \ud569\ub2c8\ub2e4.<\/li>\n
              4. \uc5d0\ud0c4\uc62c \uc140 \ud29c\ube0c\uc640 \ud314\ucf58 \ubbf8\ub514\uc5b4 \ud29c\ube0c\ub97c \ud6c4\ub4dc\uc5d0 \ub193\uc2b5\ub2c8\ub2e4.<\/li>\n
              5. 1mL\uc744 \uc0ac\uc6a9\ud558\uc138\uc694 \ub113\uc740 \uc785 \ub05d\uc744 \ucc9c\ucc9c\ud788 <\/u>\uc608\uc5f4\ub41c \ubc30\uc9c0 4mL\uc5d0 \uc138\ud3ec\ub97c \ucca8\uac00\ud569\ub2c8\ub2e4(\uc138\ud3ec \ud604\ud0c1\uc561\uc774 \uc11e\uc774\uc9c0 \uc54a\ub3c4\ub85d \uc8fc\uc758\ud558\uc138\uc694).<\/li>\n
              6. 130 rcf\uc5d0\uc11c 5\ubd84\uac04 \ud68c\uc804\uc2dc\ud0b5\ub2c8\ub2e4.<\/li>\n
              7. \uc0c1\uce35\uc561\uc744 \uc81c\uac70\ud569\ub2c8\ub2e4.<\/li>\n
              8. 2mL\uc758 PSC \ubc30\uc9c0\ub97c \ucd94\uac00\ud558\uace0 \ub113\uc740 \uc785\uad6c \ud301\uc744 \uc0ac\uc6a9\ud558\uc5ec \ub369\uc5b4\ub9ac\ub97c \ubd80\ub4dc\ub7fd\uac8c \ucabc\uac1c\ub2e4<\/u>. 6\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \ud55c \uc6f0\uc5d0 \ubc30\uc9c0\ub97c \uc62e\uae30\uace0 ROCK \uc800\ud574\uc81c(Y27632, \ucd5c\uc885 \ub18d\ub3c4 10uM) 2uL\ub97c \ucca8\uac00\ud569\ub2c8\ub2e4. \ub9c8\ud2b8\ub9ac\uc824 \ucf54\ud305 \uc6f0(6\uc6f0 \ud50c\ub808\uc774\ud2b8) \uc911 \ud558\ub098\uc5d0 \ud50c\ub808\uc774\ud2b8\ud569\ub2c8\ub2e4.<\/li>\n
              9. \uc138\ud3ec\ub97c \uac00\ubccd\uac8c \ud754\ub4e4\uc5b4 \uc138\ud3ec\ub97c \uade0\ub4f1\ud558\uac8c \ubd84\uc0b0\uc2dc\ud0a4\uace0 \uc800\uc0b0\uc18c \ubc30\uc591\uae30(5%O)\uc5d0 \ub123\uc2b5\ub2c8\ub2e4.2<\/sub>, 10% \uc8fc\uc2dd\ud68c\uc0ac2<\/sub>). \ud30c\uc885 \ud6c4 24\uc2dc\uac04 \ub3d9\uc548 \ud310\uc744 \ud754\ub4e4\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.\uba54\ubaa8: <\/strong>\ub369\uc5b4\ub9ac\uac00 \uacfc\ub3c4\ud558\uac8c \ubd80\uc11c\uc9c0\uac70\ub098 \uacf5\uaca9\uc801\uc778 \ud53c\ud3ab\ud305\uc744 \ud53c\ud558\ub294 \uac83\uc774 \ub9e4\uc6b0 \uc911\uc694\ud569\ub2c8\ub2e4. \uc774\ub294 \uc0dd\uc874\uc728\uc744 \uc0c1\ub2f9\ud788 \uac10\uc18c\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc138\ud3ec\ub294 \uc811\uc885 \uc2dc 100-300\uac1c \ud06c\uae30\uc758 \ub369\uc5b4\ub9ac\ub85c \ub0a8\uc544 \uc788\uc5b4\uc57c \ud569\ub2c8\ub2e4. \ubd80\ub4dc\ub7fd\uac8c \uc9c4\ud589\ud558\ub418, \uc138\ud3ec\uac00 \ud574\ub3d9\ub418\uba74 \ube60\ub974\uac8c \uc791\uc5c5\ud558\uc5ec \ub0c9\ub3d9 \ubcf4\ud638\uc81c\uc640 \uc811\ucd09\ud558\ub294 \uc2dc\uac04\uc744 \ucd5c\uc18c\ud654\ud558\uc2ed\uc2dc\uc624.<\/li>\n
              10. 24\uc2dc\uac04 \ud6c4 \ubc30\uc9c0\ub97c \uc81c\uac70\ud558\uace0 PSC \ubc30\uc9c0 2mL(6\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uacbd\uc6b0 1mL, 12\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uacbd\uc6b0 0.5mL)\ub97c \ucd94\uac00\ud569\ub2c8\ub2e4. hESC\/iPSC \ud504\ub85c\ud1a0\ucf5c\uc758 \uc218\ud655 \ubc0f \uad00\ub9ac\ub97c \ucc38\uc870\ud558\uc138\uc694.<\/li>\n<\/ol>\n

                PSC \ubbf8\ub514\uc5b4<\/strong><\/p>\n

                Stem Cell Technologies\uc758 mTeSR Plus(cat# 5825). \ubcf4\uad00\uc5d0 \ub300\ud55c \uacf5\uae09\uc5c5\uccb4\uc758 \uad8c\uc7a5 \uc0ac\ud56d\uc744 \ub530\ub974\uc138\uc694.<\/p>\n

                ROCK \uc5b5\uc81c\uc81c Y27632\ub780 \ubb34\uc5c7\uc785\ub2c8\uae4c?<\/strong><\/p>\n

                ROCK \uc800\ud574\uc81c Y27632\ub294 Rho \uad00\ub828 \ud0a4\ub098\uc81c p160 ROCK\uc758 \uc120\ud0dd\uc801 \uc800\ud574\uc81c\uc785\ub2c8\ub2e4. ROCK \uc800\ud574\uc81c Y27632\ub85c \uce58\ub8cc\ud558\uba74 \uc778\uac04 \ubc30\uc544\uc904\uae30\uc138\ud3ec(hESC)\uc640 \uc778\uac04 \uc720\ub3c4\ub9cc\ub2a5\uc904\uae30\uc138\ud3ec(iPSC)\uc758 \ud574\ub9ac \uc720\ub3c4 \uc138\ud3ec\uc0ac\uba78\uc774 \uc608\ubc29\ub418\uc5b4 hESC\uc640 hiPSC\uc758 \uacc4\ub300\ubc30\uc591 \ubc0f \ud574\ub3d9 \uc911\uc5d0 \uc0dd\uc874\uc728\uc774 \uc99d\uac00\ud558\uace0 \ub2e4\ub2a5\uc131\uc774 \uc720\uc9c0\ub429\ub2c8\ub2e4. ROCK \uc800\ud574\uc81c Y27632\ub294 \ub610\ud55c \ub0c9\ub3d9\ubcf4\uc874 \uc911\uc5d0 \uc904\uae30\uc138\ud3ec\uc758 \uc0dd\uc874\uc728\uc744 \ub192\uc774\ub294 \uac83\uc73c\ub85c \ub098\ud0c0\ub0ac\uc2b5\ub2c8\ub2e4. Rock \uc800\ud574\uc81c \ubd84\ucde8\uc561\uc740 \ube5b\uacfc \ubc18\ubcf5\uc801\uc778 \ub3d9\uacb0-\ud574\ub3d9 \uc8fc\uae30\uc5d0 \ubbfc\uac10\ud558\ub2e4\ub294 \uc810\uc5d0 \uc720\uc758\ud558\uc2ed\uc2dc\uc624. \uacf5\uae09\uc5c5\uccb4\uc758 \uad8c\uc7a5 \uc720\ud1b5\uae30\ud55c \ub0b4\uc5d0 \uc774 \ubd84\ucde8\uc561\uc744 \uc0ac\uc6a9\ud574\uc57c \ud569\ub2c8\ub2e4.<\/p>\n

                \uc70c\ub9ac\uc5c4 \uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac-2022<\/p>\n

                [\/et_pb_text][et_pb_text admin_label=”thawing” module_id=”thawing” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

                12. <\/strong>hESC\/iPSC \uc218\ud655 \ubc0f \uad00\ub9ac<\/strong><\/h4>\n
                  \n
                1. \uc138\ud3ec\ub97c \ud574\ub3d9\ud55c \ub2e4\uc74c \ub0a0 \ud604\ubbf8\uacbd\uc73c\ub85c \uad00\ucc30\ud558\uc5ec \uc0dd\uc874\uc728\uc744 \ud655\uc778\ud569\ub2c8\ub2e4. \ucc38\uace0: \ubd80\ucc29\ub418\uc9c0 \uc54a\uc740 \uc138\ud3ec\uac00 \ub9ce\uc774 \uad00\ucc30\ub418\ub294 \uac83\uc740 \uc815\uc0c1\uc785\ub2c8\ub2e4. \uc77c\ubd80 \uc138\ud3ec\uac00 \ubd80\ucc29\ub418\uc5b4 \uc788\ub294 \ud55c, 3~7\uc77c \uc774\ub0b4\uc5d0 \uad70\uc9d1\uc774 \uc0dd\uae38 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/li>\n
                2. \uc6f0\uc5d0\uc11c \ubc30\uc9c0\ub97c \uc81c\uac70\ud558\uace0 \uc6f0\ub2f9 2mL(6\uc6f0\uc758 \uacbd\uc6b0 1mL, 12\uc6f0\uc758 \uacbd\uc6b0 1mL, 24\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uacbd\uc6b0 0.5mL)\uc758 \uc2e0\uc120\ud558\uace0 \ub530\ub73b\ud55c PSC \ubc30\uc9c0\ub97c \ud53c\ud3ab\uc73c\ub85c \uc62e\uae41\ub2c8\ub2e4. \ud50c\ub808\uc774\ud2b8\ub97c \uc778\ud050\ubca0\uc774\ud130\ub85c \ub2e4\uc2dc \ub123\uc2b5\ub2c8\ub2e4.<\/li>\n
                3. \uc138\ud3ec\ub294 60-70%\uac00 \ud569\ub958\ud560 \ub54c\uae4c\uc9c0 \uc601\uc591\uc744 \uacf5\uae09\ubc1b\uc2b5\ub2c8\ub2e4(\uacf5\uae09\uc5c5\uccb4\uc758 \uad8c\uc7a5 \uc0ac\ud56d\uc744 \ub530\ub985\ub2c8\ub2e4).<\/li>\n
                4. 2\uc77c\ucc28\uc5d0 \uc138\ud3ec\ub97c \uad00\ucc30\ud558\uc5ec \uc131\uc7a5\ud558\uace0 \uc788\uc744 \uc218 \uc788\ub294 \ubd84\ud654\ub41c \uc138\ud3ec\ub97c \uc81c\uac70\ud574\uc57c \ud569\ub2c8\ub2e4.<\/li>\n
                5. \uc138\ud3ec\ub97c \uc138\ucc99\ud558\ub824\uba74 \ud53c\ud0b9 \ud6c4\ub4dc\ub97c \uc0ac\uc6a9\ud558\uace0 \ud53c\ud3ab \ud301\uc73c\ub85c \ubd84\ud654\ub41c \uc138\ud3ec\ub97c \uae01\uc5b4\ub0c5\ub2c8\ub2e4.<\/li>\n
                6. \uc138\ud3ec\uac00 \uc138\ucc99\ub418\uba74 \uc704\uc758 \ub2e8\uacc4\uc5d0 \ub530\ub77c \ubc30\uc9c0\ub97c \uad50\uccb4\ud569\ub2c8\ub2e4.<\/li>\n<\/ol>\n

                  (hESC\/iPSC \ub3d9\uacb0 \ub610\ub294 hESC\/iPSC \uc804\ub2ec \ucc38\uc870)<\/p>\n

                  \uba54\ubaa8:<\/strong><\/p>\n

                  PSC \ubc30\uc9c0\ub294 \uc800\uc0b0\uc18c \ud658\uacbd\uc5d0\uc11c \ub354 \ud6a8\uc728\uc801\uc778 \uac83\uc73c\ub85c \ubc1d\ud600\uc84c\uc2b5\ub2c8\ub2e4. \ub610\ud55c \uc138\ud3ec\uac00 \uc800\uc0b0\uc18c \ubc30\uc591\uae30\uc5d0\uc11c \ubc30\uc591\ub420 \ub54c \uc815\uc0c1\uc0b0\uc18c \ubc30\uc591\uae30\ubcf4\ub2e4 \ubd84\ud654\uac00 \ub35c \uc77c\uc5b4\ub098\ub294 \uac83\uc744 \uad00\ucc30\ud588\uc2b5\ub2c8\ub2e4. \ub9c8\uc9c0\ub9c9\uc73c\ub85c, \ud30c\uc885\ub41c \uc138\ud3ec\ub294 \uc800\uc0b0\uc18c \ubc30\uc591\uae30\ub97c \uc0ac\uc6a9\ud560 \ub54c \uc0dd\uc874\uc728\uc774 \ub354 \uc88b\uc2b5\ub2c8\ub2e4.<\/p>\n

                  \uc815\uc0c1\uc0b0\uc18c: 37\u00b0<\/strong>\uc528, 21% \uc6242<\/sub>, 5% \uc8fc\uc2dd\ud68c\uc0ac2<\/sub><\/p>\n

                  \uc800\uc0b0\uc18c\uc99d: 37\u00b0<\/strong>\uc528, 5%O2<\/sub>, 10% \uc8fc\uc2dd\ud68c\uc0ac2<\/sub><\/p>\n

                  \uc70c\ub9ac\uc5c4 \uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac-2022<\/p>\n

                  [\/et_pb_text][et_pb_text admin_label=”routine” module_id=”routine” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

                  13. <\/strong>hESC\/iPSC \ud1b5\uacfc<\/strong><\/h4>\n
                    \n
                  1. 6\uc6f0 \ub9c8\ud2b8\ub9ac\uc824 \ucf54\ud305 \ud50c\ub808\uc774\ud2b8\uc5d0 PSC \ubc30\uc9c0 2mL\uc744 \ub123\uace0 \ub530\ub85c \ubcf4\uad00\ud569\ub2c8\ub2e4.<\/li>\n
                  2. \ubc30\uc591\ud560 \ud50c\ub808\uc774\ud2b8\ub97c \uaebc\ub0b4\uace0 \uc6f0\uc5d0\uc11c \ubc30\uc9c0\ub97c \uc81c\uac70\ud55c \ud6c4 1mL\uc758 PBS(-\/-)\ub85c \ud55c \ubc88 \uc138\ucc99\ud569\ub2c8\ub2e4.<\/li>\n
                  3. EDTA \uc6a9\uc561 1mL\uc744 \uc6f0\uc5d0 \ub123\uace0 \uc2e4\uc628\uc5d0\uc11c 3-4\ubd84\uac04 \ubc29\uce58\ud569\ub2c8\ub2e4. \uc138\ud3ec\uac00 \ubd84\ub9ac\ub418\uae30 \uc2dc\uc791\ud560 \uc218 \uc788\uc73c\ubbc0\ub85c \ud50c\ub808\uc774\ud2b8\ub97c \uc6c0\uc9c1\uc774\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.<\/li>\n
                  4. EDTA \uc6a9\uc561\uc744 \uc81c\uac70\ud558\uace0 1mL\uc758 PSC \ubc30\uc9c0\ub97c \ucd94\uac00\ud569\ub2c8\ub2e4. EDTA\ub97c \uc138\ud3ec\uc5d0 4\ubd84 \uc774\uc0c1 \ub450\uc9c0 \ub9c8\uc2ed\uc2dc\uc624. \uc138\ud3ec\uac00 \ub4e4\uc5b4\uc62c\ub824\uc9c8 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/li>\n
                  5. \uc138\ud3ec \uc2a4\ud06c\ub808\uc774\ud37c\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc138\ud3ec\ub97c \uae01\uc5b4\ub0b4\uace0 PSC \ubc30\uc9c0\uac00 \ub4e4\uc5b4 \uc788\ub294 \ud50c\ub808\uc774\ud2b8\uc758 6\uac1c \uc6f0\uc5d0 \uc138\ud3ec\ub97c \ub098\ub215\ub2c8\ub2e4. \ucf5c\ub85c\ub2c8 \uc870\uac01\uc744 \uacfc\ub3c4\ud558\uac8c \ucabc\uac1c\uc9c0 \uc54a\ub3c4\ub85d \ud558\uace0, \ubd80\ub4dc\ub7fd\uac8c \uae01\uc5b4\ub0b4\ub3c4\ub85d \ub178\ub825\ud569\ub2c8\ub2e4. \uc138\ud3ec\ub97c \ud070 \ub369\uc5b4\ub9ac\ub85c \uc720\uc9c0\ud558\ub3c4\ub85d \ub178\ub825\ud569\ub2c8\ub2e4. \ud544\uc694\ud55c \uacbd\uc6b0 \ub113\uc740 \uc785 \ud53c\ud3ab \ud301\uc744 \uc0ac\uc6a9\ud558\uc5ec \ub369\uc5b4\ub9ac\ub97c \ucabc\uac1c\uc2ed\uc2dc\uc624. \uc138\ud3ec\ub97c \uacfc\ub3c4\ud558\uac8c \ucabc\uac1c\uba74 \uc138\ud3ec \uc0ac\ub9dd\uc774\ub098 \uacc4\ub300 \ud6c4 \uacfc\ub3c4\ud55c \uc790\ubc1c\uc801 \ubd84\ud654\uac00 \ubc1c\uc0dd\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/li>\n
                  6. 37\uc5d0\uc11c \ubc30\uc591\u00b0<\/strong>C \uac01 \uc6f0\uc5d0 \uc138\ud3ec\ub97c \uace0\ub974\uac8c \ubd84\ubc30\ud55c \ud6c4(8\uc790 \ubaa8\uc591 \ub610\ub294 L\uc790 \ubaa8\uc591 \ud754\ub4e4\uae30). \ud1b5\uacfc \ud6c4 24\uc2dc\uac04 \ub3d9\uc548 \ud50c\ub808\uc774\ud2b8 \uad50\ub780\uc744 \ud53c\ud558\uc2ed\uc2dc\uc624.<\/li>\n<\/ol>\n

                    \uba54\ubaa8<\/strong>: \uc138\ud3ec\ub97c \uae01\uc5b4\ub0b8 \ud6c4\uc5d0\ub294 \uac00\ub2a5\ud55c \ud55c \ube68\ub9ac \uc0c8\ub85c\uc6b4 \ud50c\ub808\uc774\ud2b8\ub85c \uc62e\uaca8\uc57c \ud569\ub2c8\ub2e4. \uc138\ud3ec\uac00 \ube60\ub974\uac8c(5\ubd84 \uc774\ub0b4) \ub2e4\uc2dc \ubd80\ucc29\ub418\uae30 \ub54c\ubb38\uc785\ub2c8\ub2e4.<\/p>\n

                    EDTA\ub97c \uc138\ud3ec\uc5d0 4\ubd84 \uc774\uc0c1 \ub450\uba74 \uc138\ud3ec\uac00 \ubd84\ub9ac\ub418\uae30 \uc2dc\uc791\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc774\ub7f0 \uacbd\uc6b0, 15mL \ud314\ucf58\uc5d0 4mL PSC \ubc30\uc9c0\ub97c \ub123\uace0 \uc138\ud3ec\ub97c \uc218\uc9d1\ud558\uae30\ub9cc \ud558\uba74 \ub429\ub2c8\ub2e4. \uc138\ud3ec\ub97c 130 rcf\uc5d0\uc11c 5\ubd84\uac04 \ud68c\uc804\uc2dc\ud0b5\ub2c8\ub2e4. \ud3a0\ub9bf\uc744 1mL \ubc30\uc9c0\ub85c \uc7ac\ud604\ud0c1\uc2dc\ud0a4\uace0 6\uc6f0 \ub9c8\ud2b8\ub9ac\uac94 \ucf54\ud305 \ud50c\ub808\uc774\ud2b8(\uc6f0\ub2f9 160uL)\uc5d0 \uace0\ub974\uac8c \ubd84\ubc30\ud569\ub2c8\ub2e4.<\/p>\n

                    EDTA \uc6a9\uc561: 500uL\uc758 0.5M EDTA(pH 8.0)\ub97c 500mL\uc758 DPBS(-\/-)\uc5d0 \ucca8\uac00\ud569\ub2c8\ub2e4. 0.9g\uc758 NaCl\uc744 \ucca8\uac00\ud569\ub2c8\ub2e4. \uc6a9\uc561\uc744 \uc5ec\uacfc\ud558\uc5ec \uc0b4\uade0\ud558\uace0 \ucd5c\ub300 6\uac1c\uc6d4 \ub3d9\uc548 4\u00b0C\uc5d0\uc11c \ubcf4\uad00\ud569\ub2c8\ub2e4. <\/strong><\/p>\n

                    \ub17c\ubb38\uc5d0\uc11c:<\/p>\n

                    \ud654\ud559\uc801\uc73c\ub85c \uc815\uc758\ub41c \ubc30\uc591 \uc870\uac74\uc5d0\uc11c \ud6a8\uc18c \uc5c6\ub294 \ubd84\ub9ac\ub97c \ud1b5\ud55c \uc778\uac04 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec\uc758 \ud1b5\uacfc \ubc0f \uc9d1\ub77d \ud655\uc7a5<\/strong><\/p>\n

                    \uc790\ub137 \ube44\uc5b4\uc2a4<\/a>,1<\/sup> \ub2e4\ub2c8\uc5d8 R. \uad74\ube0c\ub79c\uc2a8<\/a>,2,3<\/sup> \ub2c8\ucf5c \uc870\uc9c0<\/a>,4<\/sup>\ub85c\ub80c I. \uc2dc\ub2c8\uc2a4\uce7c\ud0a4<\/a>,1<\/sup> \uc81c\ud504\ub9ac \uc874\uc2a4<\/a>,4,5<\/sup> \uc81c\uc784\uc2a4 A. \ud1b0\uc2a8<\/a>,2,3,6<\/sup> \uadf8\ub9ac\uace0 \uad88\uce74\uc774 \uccb8<\/a>1,2<\/sup><\/p>\n

                    [\/et_pb_text][et_pb_text admin_label=”culturing” module_id=”culturing” _builder_version=”4.27.5″ custom_padding=”||||false|false” z_index_tablet=”500″ global_colors_info=”{}”]<\/p>\n

                    14. <\/strong>hESC\/iPSC \ub3d9\uacb0 <\/strong><\/h4>\n
                      \n
                    1. Bio-Cool(\uc18d\ub3c4 \uc870\uc808 \ub0c9\ub3d9\uace0)\uc744 \ucf1c\uace0 \uc628\ub3c4\ub97c -7\u00b0C\ub85c \uc870\uc815\ud558\uc138\uc694.<\/li>\n
                    2. \ubc30\uc591\uae30\uc5d0\uc11c \uc138\ud3ec\ub97c \uaebc\ub0b4 \ud604\ubbf8\uacbd\uc73c\ub85c \ubc00\uc9d1\ub3c4\uc640 \ud615\ud0dc\ub97c \uad00\ucc30\ud569\ub2c8\ub2e4.<\/li>\n
                    3. \uc6f0\uc774 70% \ud569\ub958 \uc0c1\ud0dc\ub77c\uba74 \uae30\uc874 \ubc30\uc9c0\ub97c \uc81c\uac70\ud558\uace0 PBS(-\/-)\ub85c \ud55c \ubc88 \uc138\ucc99\ud55c \ub2e4\uc74c \uc6f0\ub2f9 1 mL\uc758 EDTA \uc6a9\uc561(EDTA \uc6a9\uc561 \ud1b5\uacfc \ucc38\uc870)\uc744 \ucca8\uac00\ud569\ub2c8\ub2e4.<\/li>\n
                    4. \uc2e4\uc628\uc5d0\uc11c 3~4\ubd84\uac04 \ubc30\uc591\ud569\ub2c8\ub2e4.<\/li>\n
                    5. EDTA \uc6a9\uc561\uc744 \ud761\uc778\ud558\uace0 \ucc28\uac00\uc6b4 mFreSR \ubc30\uc9c0(cat#05855, Stem Cell Technologies) 1 mL\ub97c \ucca8\uac00\ud569\ub2c8\ub2e4.<\/li>\n
                    6. \uc138\ud3ec \uc2a4\ud06c\ub808\uc774\ud37c\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc138\ud3ec\ub97c \ubd80\ub4dc\ub7fd\uac8c \ub4e4\uc5b4\uc62c\ub9bd\ub2c8\ub2e4. \uc138\ud3ec\ub97c \uac00\ub2a5\ud55c \ud55c \ud070 \ub369\uc5b4\ub9ac\ub85c \uc720\uc9c0\ud558\uace0 \uc704\uc544\ub798\ub85c \ud53c\ud3ab\ud305\ud558\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.<\/li>\n
                    7. \ub113\uc740 \uc785 \ub05d\uc744 \uc0ac\uc6a9\ud558\uc5ec \uc138\ud3ec\/mFreSR\uc744 \ud06c\ub77c\uc774\uc624\ud29c\ube0c\ub85c \uc62e\uae41\ub2c8\ub2e4. 8\ub2e8\uacc4\ub97c \uc900\ube44\ud560 \ub54c\uae4c\uc9c0 \ubc14\uc774\uc54c\uc744 \uc5bc\uc74c \uc704\uc5d0 \ub450\uc2ed\uc2dc\uc624.<\/li>\n
                    8. \ud29c\ube0c\ub97c Bio-Cool\uc5d0 \ub123\uace0 10\ubd84 \ub3d9\uc548 \ubc30\uc591\ud569\ub2c8\ub2e4.<\/li>\n
                    9. \uc561\uccb4\uc9c8\uc18c\ub97c \uad6c\ud558\uc138\uc694.<\/li>\n
                    10. 10\ubd84 \ud6c4, \uc8fc\uac71\uc744 \uc561\uccb4 \uc9c8\uc18c\uc5d0 \ub2f4\uadf8\uace0 \ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c\uc758 \uce21\uba74\uc744 \uc57d 10~30\ucd08 \ub3d9\uc548 \ub9cc\uc9c0\uac70\ub098 \ud06c\ub77c\uc774\uc624\ubc14\uc774\uc54c \uce21\uba74\uc5d0 \uacb0\uc815\uc774 \ud615\uc131\ub420 \ub54c\uae4c\uc9c0 \ub9cc\uc838\uc11c \uc138\ud3ec\ub97c \uc811\uc885\ud569\ub2c8\ub2e4.<\/li>\n
                    11. "PROG" \ubc84\ud2bc\uc744 \ub20c\ub7ec \ud504\ub85c\uadf8\ub7a8 1\uc744 \uc2dc\uc791\ud558\uace0 \ubc84\ud2bc\uc744 \ub2e4\uc2dc \ub20c\ub7ec \ud504\ub85c\uadf8\ub7a8\uc744 \uc9c4\ud589\ud558\uba74 0.5\u00b0C\/\ubd84\uc758 \uc18d\ub3c4\uac00 \ud45c\uc2dc\ub418\uc5b4\uc57c \ud569\ub2c8\ub2e4. \uadf8\ub7f0 \ub2e4\uc74c "RUN"\uc744 \ub204\ub985\ub2c8\ub2e4.<\/li>\n
                    12. \uc628\ub3c4\uac00 -65\u00b0C\uc5d0 \ub3c4\ub2ec\ud558\uba74 \ud06c\ub77c\uc774\uc624\ud29c\ube0c\ub97c \uc561\uccb4 \uc9c8\uc18c\ub85c \uc62e\uaca8 \ubcf4\uad00\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/li>\n<\/ol>\n

                      \ub300\uc548<\/p>\n

                      \ub610 \ub2e4\ub978 \uc635\uc158\uc740 \ub0c9\ub3d9 \uc6a9\uae30(Biocision-CoolCell)\uc5d0 \ud06c\ub77c\uc774\uc624 \ud29c\ube0c\ub97c \ub123\uace0 \ubc24\uc0c8 -80\u00b0C\uc5d0\uc11c \ubcf4\uad00\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \ub2e4\uc74c\ub0a0 \ud06c\ub77c\uc774\uc624 \ud29c\ube0c\ub97c \uc561\uccb4 \uc9c8\uc18c(\uc561\uc0c1 \ub610\ub294 \uc99d\uae30\uc0c1)\ub85c \uc62e\uae41\ub2c8\ub2e4.<\/p>\n

                      \uc70c\ub9ac\uc5c4 \uc2a4\ud0e0\ud3ec\ub4dc \ubc15\uc0ac-2022<\/p>\n

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                      \uac00\uc785\ud558\uae30<\/h2>\n

                      \uc6b0\ub9ac\ub97c \uc704\ud574<\/h2>\n

                      \uc5c5\ub370\uc774\ud2b8!<\/h2>\n

                      [\/et_pb_cta][\/et_pb_column][et_pb_column type="1_4" _builder_version="4.16" custom_padding="|||" global_colors_info="{}" custom_padding__hover="|||"][et_pb_cta button_url="https:\/\/progeriaresearch.donorsupport.co\/-\/XZHJVWZR" button_text="\uc9c0\uae08 \uae30\ubd80\ud558\uae30" admin_label="\ud568\uaed8\ub77c\uba74 \uc6b0\ub9ac\ub294 \uce58\ub8cc\ubc95\uc744 \ucc3e\uc744 \uac83\uc785\ub2c8\ub2e4!" module_class="sign-btn" _builder_version="4.16" header_font_size="25px" background_color="#29327a" animation_style="slide" animation_direction="left" animation_intensity_slide="25%" header_font_size_tablet="" header_font_size_phone="30px" header_font_size_last_edited="on|desktop" body_font_size_tablet="" body_font_size_phone="" body_font_size_last_edited="on|desktop" z_index_tablet="500" border_radii="on|25px|25px|25px|25px" global_colors_info="{}" button_bg_color__hover_enabled="on" \ubc84\ud2bc_bg_color__hover="#8fd2ed" \ubc84\ud2bc_\ud14c\ub450\ub9ac_\uc0c9\uc0c1__hover_\ud65c\uc131\ud654="\ucf1c\uc9d0"]<\/p>\n

                      \uc6b0\ub9ac\ub294 \ud568\uaed8<\/h2>\n

                      \ud560 \uac83\uc774\ub2e4<\/em><\/h2>\n

                      \uce58\ub8cc\ubc95\uc744 \ucc3e\uc544\ub77c!<\/h2>\n

                      [\/et_pb_cta][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2026\/03\/2026-footer-image-copy.png” title_text=”2026 footer image copy” _builder_version=”4.27.5″ _module_preset=”default” custom_margin=”35px||||false|false” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"

                      [et_pb_section fb_built="1" \uc804\uccb4 \ub108\ube44="\ucf1c\uae30" \ube44\ud65c\uc131\ud654\ub428="\uaebc\uc9d0|\uaebc\uc9d0|\uaebc\uc9d0" _\ube4c\ub354_\ubc84\uc804="4.16" \ud14c\ub450\ub9ac_\ub108\ube44_\uc544\ub798="55px" \ud14c\ub450\ub9ac_\uc0c9\uc0c1_\uc544\ub798="#29327a" \uc7a0\uae40="\uaebc\uc9d0" \uae00\ub85c\ubc8c_\uc0c9\uc0c1_\uc815\ubcf4="{}"][et_pb_\uc804\uccb4 \ub108\ube44_\ud5e4\ub354 _\ube4c\ub354_\ubc84\uc804="4.16" \uc81c\ubaa9_\uae00\uaf34="||||||||" \uc81c\ubaa9_\uae00\uaf34_\ud06c\uae30="55" \ubc30\uacbd_\uc0c9\uc0c1="#29327a" \ubc30\uacbd_\uc774\ubbf8\uc9c0="https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/12\/Jes.jpg" \ubc30\uacbd_\uc704\uce58="center_left" \uc0ac\uc6a9\uc790 \uc9c0\uc815_\ud328\ub529="9vw|0px|9vw||true|" custom_padding_tablet="" custom_padding_phone="|56px||" custom_padding_last_edited="on|desktop" title_font_size_tablet="45px" title_font_size_phone="40px" title_font_size_last_edited="on|phone" z_index_tablet="500" custom_css_main_element="background-position: center 18% !important;" global_colors_info="{}"] \uc720\ub3c4 \ub2e4\ub2a5\uc131 \uc904\uae30 \uc138\ud3ec [\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built="1" use_custom_gutter="on" gutter_width="1" specialty="on" padding_left_1="35px" padding_left_2="35px" padding_2_tablet="|||0px" padding_2_phone="" padding_2_last_edited="on|desktop" module_class_1="sidebar-secondary-nav" module_class="handprint-bg" _builder_version="4.16" background_image="https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2019\/04\/blue-handprint-only.png" parallax="on" parallax_method="off" \ub0b4\ubd80_\ub108\ube44="100%" \ub0b4\ubd80_\ucd5c\ub300_\ub108\ube44="100%" \uc0ac\uc6a9\uc790 \uc815\uc758_\ud328\ub529="0|0px|54px|0px|false|false" z_index_tablet="500" \ud14c\ub450\ub9ac_\ub108\ube44_\uc704\ucabd="10px" \ud14c\ub450\ub9ac_\uc0c9\uc0c1_\uc704\ucabd="#8fd2ed" [\u2026]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\t\t\t\t[vc_row][vc_column][vc_custom_heading text=\"Induced Pluripotent Stem Cells\" font_container=\"tag:h1|text_align:center\" use_theme_fonts=\"yes\"][vc_column_text]\r\n\r\n\r\n\r\n
                      \"\"<\/td>\r\n<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\nThe Progeria Research Foundation Cell & Tissue Bank\r\n<\/strong>Human Induced Pluripotent Stem Cells (iPSC)\r\n
                        \r\n \t
                      1. Progeria iPSCs <\/strong>Background Information for the Non-scientist<\/strong><\/a>\u00a0<\/strong><\/li>\r\n \t
                      2. Purpose of <\/strong>Induced Pluripotent Stem Cell Generation and Distribution by The Progeria Research Foundation\u00a0<\/strong>\u00a0<\/a><\/li>\r\n \t
                      3. Generation of Hutchinson-Gilford Progeria Syndrome Induced-Pluripotent Stem Cells (iPSCs)<\/strong>\u00a0<\/a><\/li>\r\n \t
                      4. Quality Control: Validation and Characterization<\/strong>\u00a0<\/a><\/li>\r\n \t
                      5. Original Starting Material from which iPSCs Were Derived<\/strong><\/a><\/li>\r\n \t
                      6. Join our Email List for Future iPSC Updates and New Cell Lines<\/strong>\u00a0\u00a0<\/strong><\/a><\/li>\r\n \t
                      7. Questions?\u00a0 Contact us.<\/strong><\/a><\/li>\r\n \t
                      8. Ordering iPSC Lines<\/strong><\/a>\u00a0<\/strong><\/li>\r\n \t
                      9. HGPS and Control iPSC Culture Media Preparation<\/strong>\u00a0<\/strong><\/a><\/li>\r\n \t
                      10. Preparing Madrigal Plates\u00a0<\/strong><\/a><\/li>\r\n \t
                      11. Thawing hESCs and iPSCs<\/strong><\/a><\/li>\r\n \t
                      12. Harvesting and Caring for hESCs and iPSCs<\/strong>\u00a0<\/strong><\/a><\/li>\r\n \t
                      13. Passaging with EDTA solution<\/strong><\/a><\/li>\r\n \t
                      14. Freezing hESCs and iPSCs<\/a><\/strong><\/li>\r\n<\/ol>\r\n\r\n
                        \r\n\r\n                        <\/a>1. iPSC Background information for the non-scientist<\/strong>\r\nStem cells are \u201cimmature\u201d cells that have not yet committed to becoming any one cell type.\u00a0 They are pliable because they have the potential to develop into many different types of mature cells in the body, such as cells that make up the heart or blood vessels, and other tissues and organs.\u00a0 In 2007, researchers discovered a strategy for creating stem cells in the laboratory by reprogramming mature adult cells that we commonly grow for research purposes.1, 2<\/sup><\/strong> . These artificially created stem cells are called Induced Pluripotent Stem Cells (\u201ciPSCs\u201d). For the field of Progeria, this is a huge breakthrough.\u00a0 For the first time, scientists can now make Progeria stem cells and ask questions about how stem cells function and develop in Progeria.\u00a0 Previously there was no source of human Progeria stem cells, and there was therefore a void of information about how Progeria stem cells function compared with stem cells from people without Progeria.\u00a0 In addition, scientists can re-program the Progeria stem cells to create, for the first time, mature Progeria blood vessels, heart cells, and other cell types.\u00a0 Until now, there was no source of human Progeria heart or blood vessel cells.\u00a0 We can now ask key <\/strong>questions about the heart disease that leads to early death in Progeria from heart attacks and strokes. We can compare these discoveries with the heart disease and aging in the general population and discover more about what influences aging in all of us.\u00a0 Already there have been several excellent studies published using Progeria stem cells.3-5<\/sup> \u00a0Our goal at The Progeria Research Foundation is to facilitate many more discoveries using this invaluable tool.\u00a0 For a primer on stem cells, please see this US government website:\u00a0https:\/\/stemcells.nih.gov\/info\/basics.htm<\/a>\r\n
                        \r\n
                          \r\n \t
                        1. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. <\/em>2007;131:861-872.<\/li>\r\n \t
                        2. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin, II, Thomson JA. Induced pluripotent stem cell lines derived from human somatic cells. Science. <\/em>2007;318:1917-1920.<\/li>\r\n \t
                        3. Liu GH, Barkho BZ, Ruiz S, Diep D, Qu J, Yang SL, Panopoulos AD, Suzuki K, Kurian L, Walsh C, Thompson J, Boue S, Fung HL, Sancho-Martinez I, Zhang K, Yates J, 3rd, Izpisua Belmonte JC. Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome. Nature. <\/em>2011;472:221-225.<\/li>\r\n \t
                        4. Misteli T. HGPS-derived iPSCs for the ages. Cell Stem Cell. <\/em>2011;8:4-6.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/a>2.\u00a0Purpose of induced pluripotent stem cell (iPSC) generation and distribution by The Progeria Research Foundation<\/strong>\r\nThe mission of The Progeria Research Foundation is to discover treatments and the cure for Hutchinson-Gilford Progeria Syndrome and its aging-related disorders. In 2009, PRF entered into a collaboration with an expert team of scientists at the University of Toronto, Canada, under the direction of William Stanford, PhD, to generate high quality Progeria iPSCs. Dr. Stanford is the Canada Research Chair in Integrative Stem Cell Biology. As of 2011, PRF continues to collaborate with Dr. Stanford at the University of Ottawa, Canada where he is Professor of Cellular and Molecular Medicine, Faculty of Medicine, and Senior Scientist at Ottawa Hospital Research Institute's Sprott Centre for Stem Cell Research.<\/a>\r\n\r\nOur goal is to provide this invaluable tool to researchers throughout the world.\u00a0 This new research tool will be used to generate new and innovative research in Progeria, as well as its relationship to heart disease and aging. \u00a0<\/strong>\r\n\r\n3. Generation of Hutchinson-Gilford Progeria Syndrome Induced-Pluripotent Stem Cells (iPSCs)<\/strong><\/a>\r\nInduced-Pluripotent Stem Cells (iPSCs) were derived using VSVG-pseudotyped retroviral transduction of four human factors, Oct4, Sox2, Klf4, and c-Myc into fibroblasts. \u00a0iPSC colonies were derived on mouse-embryonic fibroblasts (MEFs). The procedure used was essentially as previously described but without the use of the EOS reporter (Nature Protocols 4: 1828-1844, 2009). \u00a0<\/strong>\r\n\r\n4. Quality Control: Validation and Characterization<\/strong>\r\nThe lines that are currently available have undergone several validation steps (see downloadable PDFs below):\r\n
                          \r\n
                            \r\n \t
                          1. Mycoplasma Testing for each line: Dr. Stanford\u2019s lab has performed mycoplasmaanalysis by PCR for each cell line.\u00a0 In addition, after expansion and prior to shipping cells, the lines will be retested for mycoplasma.<\/li>\r\n \t
                          2. Immunostaining for pluripotency markers Tra-1-60, Tra-1-81, and SSEA4.<\/li>\r\n \t
                          3. Alkaline Phosphatase Staining as an indicator of pluripotency<\/li>\r\n \t
                          4. Embryoid body formation and subsequent immunostaining for markers of the three germ-layers. Markers tested were \u03b2III-Tubulin (Ectoderm), Smooth-Muscle Actin (Mesoderm), and Gata4 or AFP (Endoderm)<\/li>\r\n \t
                          5. Karyotype analysis.<\/li>\r\n \t
                          6. Re-expression of lamin A in differentiated cells<\/li>\r\n \t
                          7. Teratoma assays<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/a>\r\nAdditional validation in process:<\/strong>\r\nSome lines have completed teratoma assays as shown in supporting data. For all other lines, teratoma assays are in process and status will be updated as these assays are completed.\r\n\r\n5.\u00a0\u00a0 Original starting material from which these iPS cells were derived<\/strong>\r\niPSCs were derived from PRF Cell & Tissue Bank non-transformed fibroblast cell lines.\r\n\r\nThe transduction method used for all iPS lines was Retrovirus MKOS.\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
                            \r\n
                            iPSC Line<\/strong> ID<\/strong>\r\n<\/strong><\/div><\/td>\r\n
                            		\r\n
                            Mutation<\/strong>\r\n<\/strong><\/div><\/td>\r\n
                            		\r\n
                            Gender<\/strong> and Donation<\/strong> Age\r\n<\/strong><\/div><\/td>\r\n
                            		\r\n\r\n
                            Supporting Data\u00a0\"\"<\/strong><\/div><\/td>\r\n<\/tr>\r\n
                            \r\n
                            HGADFN003 iPS 1B<\/div><\/td>\r\n
                            		\r\n
                            \u00a0LMNA<\/em>Exon 11,\r\n1824 C>T<\/div><\/td>\r\n
                            		\r\n
                            Male 2yr 0mo<\/div><\/td>\r\n
                            		\r\n
                            \u00a0<\/strong>Dermal Fibroblasts\r\nHGADFN003<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGADFN003 iPS 1C<\/div><\/td>\r\n
                            		\r\n
                            \u00a0LMNA<\/em> Exon 11,\r\n1824 C>T<\/div><\/td>\r\n
                            		\r\n
                            Male 2yr 0mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts\r\nHGADFN003<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGDFN003\r\niPS 1D<\/div><\/td>\r\n
                            		\r\n
                            \u00a0LMNA <\/em>Exon 11,\r\n1824 C>T<\/div><\/td>\r\n
                            		\r\n
                            Male 2yr 0mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts\r\nHGADFN003<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGADFN167 iPS 1J<\/div><\/td>\r\n
                            		\r\n
                            \u00a0LMNA <\/em>Exon 11, 1824 C>T<\/div><\/td>\r\n
                            		\r\n
                            Male 8yr 5mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts HGADFN167<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGADFN167 iPS 1Q<\/div><\/td>\r\n
                            		\r\n
                            \u00a0LMNA<\/em> Exon 11, 1824 C>T<\/div><\/td>\r\n
                            		\r\n
                            Male 8yr 5mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts HGADFN167<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGMDFN090 iPS 1B<\/div><\/td>\r\n
                            		\r\n
                            \u00a0<\/em>Mother of HGADFN167 (unaffected)<\/div><\/td>\r\n
                            		\r\n
                            Female 37yr 10mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts\r\nHGMDFN090<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGMDFN090 iPS 1C<\/div><\/td>\r\n
                            		\r\n
                            \u00a0<\/em>Mother of HGADFN167 (unaffected)<\/div><\/td>\r\n
                            		\r\n
                            Female 37yr 10mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts\r\nHGMDFN090<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGFDFN168 iPS1 D2<\/div><\/td>\r\n
                            		\r\n
                            \u00a0Father of HGADFN167 (unaffected)<\/div><\/td>\r\n
                            		\r\n
                            Male 40yr\r\n5mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts HGFDFN168<\/div><\/td>\r\n
                            		\r\n
                            \r\n
                            HGFDFN168 iPS1P<\/div><\/td>\r\n
                            		\r\n
                            Father of HGADFN167 (unaffected)<\/div><\/td>\r\n
                            		\r\n
                            Male 40yr<\/div>\r\n
                            5mo<\/div><\/td>\r\n
                            		\r\n
                            Dermal Fibroblasts\r\nHGFDFN168<\/div><\/td>\r\n
                            		<\/a>\r\n
                            168 iPS1P<\/a><\/div><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n
                            <\/div>\r\n\"\"<\/a>\u00a0PRF AVAILABLE CELL LINES<\/a><\/strong>\u00a0<\/strong>\r\n\r\n6.\u00a0Join our email list for future iPSC updates and new cell lines<\/strong>\r\nWe are continuing to generate iPSC lines.\u00a0 If you would like periodic updates on iPSCs held in the PRF Cell & Tissue Bank,\u00a0please join our emailing list by clicking here<\/a><\/strong>\r\n\r\n7.\u00a0Questions?<\/strong><\/a>\r\nPlease contact Leslie Gordon, MD, PhD, Medical Director, with any questions or needs, at lgordon@progeriaresearch.org<\/a> or 978-535-2594\r\n<\/strong>\r\n\r\n8.\u00a0 Ordering iPS cell lines<\/strong>\r\n\r\nIn 2014, PRF instituted a policy of no changes to our MTA. This is the result of 12 years of contractual arrangements with 70 research teams working at institutions in 14 countries. PRF and its counsel have taken into consideration the issues that have arisen in that time period and edited the agreement accordingly, resulting in what we feel are fair and reasonable terms.\r\n\r\nFor U.S. Federal Government Institutions or questions, please contact<\/span>\u00a0<\/span>Wendy Norris at:\u00a0<\/span>wnorris@lifespan.org<\/a><\/span>\u00a0or\u00a0401-274-1122 x 48063<\/a>.\r\n\r\nStep 1: Complete an application and material transfer agreement\r\n<\/strong>\r\n<\/a>\u00a0 Application and Agreement for Non-government Institutions<\/a>\r\n\r\n\u00a0Material Transfer Agreement for Non-government Institutions*<\/a>\r\n\r\nStep 2: Return the completed application and material transfer agreement to PRF at info@progeriaresearch.org<\/a>.\u00a0 Once approved, you will receive an email confirming your order and anticipated shipping date.\u00a0<\/strong><\/a>\r\n\r\nStep 3: <\/strong>Dr. Stanford\u2019s laboratory is currently distributing lines\u00a0in frozen cryovials.\u00a0 His laboratory will email you when the culture has been shipped, with shipping and tracking information. Inexperienced researchers are directed to obtain training at specialized courses essential to human embryonic stem cell\/iPSCs work.<\/a>\r\n\r\nStep 4: The University of Ottawa will charge $84.00 per iPSC line plus courier costs, if any, and will send you a bill directly.<\/strong>\r\n\r\n9.\u00a0 HGPS and Control iPS Cell Culture Media Preparation<\/strong>\r\niPSC and ESCs need to be fed with either TeSR-E8 from Stem Cell\u00a0Technologies (cat# 05990) or Essential 8 medium from ThermoFisher Scientific (cat#A1517001). Please follow the supplier's recommendations for storage.\r\n\r\n10.Preparing Madrigal Plates<\/strong>\r\n\r\nNote<\/strong>: All steps involving matrigel should be done as quickly as possible and stay as cold as possible.\r\n
                              \r\n \t
                            1. Thaw matrigel bottle (10ml) at 4C.<\/li>\r\n \t
                            2. Take matrigel bottle and add 10ml of cold DMEM\/F12 media.<\/li>\r\n \t
                            3. Aliquot 1ml of matrigel in pre-chilled 15ml falcon tubes.<\/li>\r\n \t
                            4. Freeze all aliquots at -20C<\/li>\r\n \t
                            5. To make matrigel plate, remove one aliquot of matrigel (1ml) from -20C and add 18ml of cold DMEM\/F12. Let pellet thaw and once thawed mix well (without creating bubbles) and plate (1ml\/well for 6 well plate, 0.5ml\/well for 12 well plate, 0.25ml\/well for 24 well plate). These plates are labelled as MG1.<\/li>\r\n \t
                            6. If using plate immediately, let plate sit at room temperature for 1 hour, observe matrigel under the microscope. Matrigel should be well dispersed and not \u201cclumpy\u201d.<\/li>\r\n \t
                            7. Once 1 hour is passed transfer matrigel to a second plate (this plate is labelled MG2). If using right away, incubate at room temperature for 1 hour.<\/li>\r\n \t
                            8. If using plates at another time, wrap edge of plate with paraffin and store at 4 degrees.<\/li>\r\n<\/ol>\r\n<\/a>\r\nMatrigel \u2013 BD\/Fisher, cat#CB-40230\r\nDMEM\/F12 \u2013 Life Technologies, cat#11330-057\r\n\r\nDr. William Stanford-2018\r\n\r\n11. Thawing hESCs and iPSCs<\/strong>\r\n\r\nThawing ES or iPS cells (per cryo-vial)<\/u><\/strong>\r\n
                                \r\n \t
                              1. Take matrigel plate out of 4C and warm at room temperature for one hour or make fresh matrigel plate (see preparing matrigel plate protocol<\/strong>).<\/li>\r\n \t
                              2. Warm 4ml of PSC media (pluripotent stem cell media) in a 15ml falcon tube.<\/li>\r\n \t
                              3. Remove cells from freezer and swirl in 37 degree water bath until only a small ice chunk is left. This step must be done quickly.<\/li>\r\n \t
                              4. Ethanol cell tube and falcon media tube and place in the hood.<\/li>\r\n \t
                              5. Use a 1ml wide mouth tip to slowly add cells to 4ml of prewarmed media<\/li>\r\n \t
                              6. Spin at 850rpm for 5min<\/li>\r\n \t
                              7. Remove supernatant<\/li>\r\n \t
                              8. Add 2ml of PSC media and with a\u00a0wide mouth tip break up clumps. Transfer media to one well of a 6 well plate add\u00a02ul of\u00a0ROCK inhibitor (Y27632, final concentration of 10uM)\u200b.<\/li>\r\n \t
                              9. Remove media after 24 hours and add 2ml of PSC media (for a 6 well, 1ml for 12 well and 0.5ml for 24 well plate). See Harvesting and Caring for hESC\/iPSC <\/strong>protocol<\/li>\r\n<\/ol>\r\nPSC media\r\n<\/u><\/strong>TeSR-E8 from Stem Cell Technologies, catalogue # 05990 or Essential 8 Medium from ThermoFisher Scientific, catalogue # A1517001\r\n\r\nWhat is ROCK Inhibitor Y27632?<\/a>\r\n<\/u><\/strong>ROCK Inhibitor Y27632 is a selective inhibitor of the Rhoassociated kinase p160ROCK. Treatment with ROCK Inhibitor Y27632 prevents dissociationinduced apoptosis of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC), increasing the survival rate and maintaining pluripotency during subcultivation and thawing of hESCs and hiPSCs. ROCK Inhibitor Y27632 also has been shown to enhance the survival rate of stem cells during cryopreservation.\r\n\r\nDr. William Stanford-2018\r\n\r\n12.Harvesting and Caring for hESCs and iPSCs<\/strong>\r\n
                                  \r\n \t
                                1. Remove media from 4C and place in 37C water bath.<\/li>\r\n \t
                                2. The day after cells were thawed take a look at them under the microscope to determine survival rate. (If cells were frozen only with mFreSR the survival rate is appr. 20%, if cells were frozen using the Bio-Cool survival rate is appr. 60%).<\/li>\r\n \t
                                3. Remove media from the wells and pipette 2ml (for a 6 well, 1ml for 12 well and 0.5ml for 24 wells plate) of fresh PSC media per well.<\/li>\r\n \t
                                4. Place plate in 37C incubator (hypoxic condition: 10% CO2 and 5% O2).<\/li>\r\n \t
                                5. Cells are fed every day for the next 3-5 days until 80% confluent.<\/li>\r\n \t
                                6. Cells should be observed and cleaned of any differentiated cells that might be growing.<\/li>\r\n \t
                                7. To clean cells use the picking hood and \u201cscrap off\u201d differentiated cells with a pipette tip.<\/li>\r\n \t
                                8. Once cells are cleaned changed media as done in above steps.<\/li>\r\n<\/ol>\r\n(See Freezing hESC\/iPSC or Passing hESC\/iPSC<\/strong>)\r\n\r\nNOTE:\r\n<\/u><\/strong>PSC media was determine to be more efficient\u00a0 in a hypoxic environment. We have also observed that less differentiation occurs when cells are grown in hypoxic incubators versus normoxic. Also when seeding cells there\u2019s a better survival rate when using a hypoxic incubator.<\/a>\r\n\r\nNormoxic: 5% CO2\r\nHypoxic: 5%O2, 10% CO2\r\n\r\nDr. William Stanford-2018\r\n\r\n13.Passaging with EDTA solution<\/strong>\r\n
                                    \r\n \t
                                  1. Add 2ml of PSC media to a 6 well matrigel coated plate and set aside.<\/li>\r\n \t
                                  2. Take the plate to be passaged and remove the media from the well and wash once with 1ml of PBS(-\/-).<\/li>\r\n \t
                                  3. Add 1ml of\u00a0 the EDTA solution to the well and leave for 3-4min at room temperature. \u00a0Don\u2019t move the plate around as cells can start detaching.<\/li>\r\n \t
                                  4. Once 4 min. is up remove EDTA solution and add 1ml of PSC media. Do not leave EDTA on the cells for more than 4 min as this will cause the cells to lift off.<\/li>\r\n \t
                                  5. Scrape cells (with cell scrapper) and divide cells amongst the 6 wells of your plate containing PSC media (I\u2019ve been taking 160ul into each well). Avoid breaking up the pieces in very small pieces. Try to keep large chunks. Preferably use a wide mouth pipette tip.<\/li>\r\n<\/ol>\r\nNOTE<\/u><\/strong>: Once the cells have been scraped you want to transfer them to the new plate as soon as possible because the cells will reattach quickly (within 5min).\r\n\r\nIf EDTA is left on the cells for more than 4mins. the cells can start to detach. If this happen simply scrap cells and collect in a 15ml falcon with 4ml of PSC media. Spin cells at 850rpm for 5 min. Resuspend the pellet with 1ml of media and divide evenly among a 6 well matrigel coated plate (160ul per well).\r\n\r\nEDTA solution<\/u><\/strong>:\u00a0 Add 500ul of 0.5M EDTA (pH 8.0) into 500ml of DPBS (-\/-). Add 0.9g of NaCl and adjust the osmolarity to 340 mOsm. Filter the solution to sterilize and store it at 4C for up to 6 months. We want the least amount of disturbance for the cells during dissociation therefore the EDTA solution is at the same osmolarity as the PSC media. <\/u><\/strong>\r\n\r\nFrom the paper:<\/a>\r\nPassaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions\r\n<\/strong>Jeanette Beers<\/a>,1<\/sup>\u00a0Daniel R. Gulbranson<\/a>,2,3<\/sup>\u00a0Nicole George<\/a>,4<\/sup>Lauren I. Siniscalchi<\/a>,1<\/sup>\u00a0Jeffrey Jones<\/a>,4,5<\/sup>\u00a0James A. Thomson<\/a>,2,3,6<\/sup>\u00a0and\u00a0Guokai Chen<\/a>1,2<\/sup>\r\n\r\n14.Freezing hESCs and iPSCs<\/strong>\r\n
                                      \r\n \t
                                    1. Turn on Bio-Cool (Controlled Rate Freezer) and adjust SP temperature to -7C.<\/li>\r\n \t
                                    2. Remove cells from incubator and observe confluency and morphology under the microscope.\r\nNOTE:<\/strong> Looking for ~80% confluency<\/li>\r\n \t
                                    3. If confluent, remove old media and wash once with PBS(-\/-) then add 1 ml of EDTA solution (see passaging with EDTA solution) per well.<\/li>\r\n \t
                                    4. Incubate at room temperature for 3-4 minutes.<\/li>\r\n \t
                                    5. Aspirate the EDTA solution and add 1 ml of mFreSR media (cat#05855, Stem Cell Technologies)<\/li>\r\n \t
                                    6. Use cell scraper to detach the cells from the well (gently).<\/li>\r\n \t
                                    7. Transfer cells\/mFreSR to a cryotube using a wide mouth tip.<\/li>\r\n \t
                                    8. Place tubes in Bio-Cool and incubate for 10min.<\/li>\r\n \t
                                    9. Get liquid nitrogen.<\/li>\r\n \t
                                    10. After 10min. seed the cells by dipping a spatula into liquid nitrogen and touching the side of the cryo-vial for approximately 10 seconds or until you see a crystal form on the side of the cryo-vial.<\/li>\r\n \t
                                    11. Start program 1 by pushing \u201cPROG\u201d button and going through the program by pushing the button again and you should see the rate of 0.5C\/min. , then press \u201cRUN\u201d.<\/li>\r\n \t
                                    12. Once temperature reaches -65C the cryo-tubes can be transferred and stored in liquid nitrogen.<\/li>\r\n<\/ol>\r\nAlternatives\r\n<\/u><\/strong>1-Another option is to place the cryo-tubes in a freezing container (Biocision-CoolCell) and store at -80C overnight. The next day store cryo-tubes in liquid nitrogen.\r\n\r\nDr. William Stanford-2018[\/vc_column_text][\/vc_column][\/vc_row]\t\t","_et_gb_content_width":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-1010","page","type-page","status-publish","hentry"],"yoast_head":"\nInduced | The Progeria Research Foundation<\/title>\n<meta name=\"description\" content=\"To learn more about Induced Pluripotent Stem Cells, please contact Leslie Gordon, MD, PhD, Medical Director at lgordon@progeriaresearch.org.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.progeriaresearch.org\/ko\/induced-pluripotent-stem-cells\/\" \/>\n<meta property=\"og:locale\" content=\"ko_KR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Induced | The Progeria Research Foundation\" \/>\n<meta property=\"og:description\" content=\"To 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