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RPMI 1640 \u2013 TermoFisher # 11875
15% Soro Fetal Bovino (FBS) \u2013 ThermoFisher #10437-028
1% (1X) Penicilina-Estreptomicina (opcional) \u2013 ThermoFisher #15140-122<\/p>\n
As c\u00e9lulas congeladas ser\u00e3o recebidas em al\u00edquotas de 1 ml em gelo seco. Mantenha as c\u00e9lulas congeladas at\u00e9 descongelar para cultura celular ou coloque em armazenamento de nitrog\u00eanio l\u00edquido se n\u00e3o iniciar as culturas imediatamente. No entanto, \u00e9 recomendado que, se voc\u00ea for armazenar as c\u00e9lulas para uso posterior, cultive estoques frescos e congele v\u00e1rias ampolas. As c\u00e9lulas s\u00e3o criopreservadas em 45% RPMI-1640, 50% FBS e 5% DMSO (grau de cultura de tecidos).<\/p>\n
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Professor de Pesquisa Pedi\u00e1trica Warren Alpert Medical School da Brown University e Departamento de Pediatria, Hasbro Children's Hospital, Providence, RI Departamento de Anestesia, Children's Hospital Boston e Harvard Medical School, Boston, MA Diretor M\u00e9dico, The Progeria Research Foundation<\/p>\n
Telefone: 978-535-2594 [\/et_pb_text][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”3_4″ _builder_version=”4.16″ custom_padding=”|||20px” custom_padding_tablet=”20px|||0px” custom_padding_phone=”” custom_padding_last_edited=”on|phone” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.27.2″ custom_padding=”||0px|||” custom_padding_last_edited=”off|desktop” global_colors_info=”{}”]<\/p>\n [\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=\u201d1\u2033 module_class=\u201drodap\u00e9\u201d _builder_version=\u201d4.21.0\u2033 background_color=\u201d#29327a\u201d custom_margin=\u201d-2px|||||\u201d custom_padding=\u201d0|0px|0|0px|false|false\u201d z_index_tablet=\u201d500\u2033 border_width_top=\u201d12px\u201d border_color_top=\u201d#00b2e2\u2033 global_module=\u201d133\u2033 locked=\u201doff\u201d global_colors_info=\u201d{}\u201d][et_pb_row_inner 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c\u00f3pia de tira de rodap\u00e9\u201d _builder_version=\u201d4.27.0\u2033 _module_preset=\u201ddefault\u201d custom_margin=\u201d35px||||false|false\u201d global_colors_info=\u201d{}\u201d][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":" [et_pb_section fb_built=\u201d1\u2033 largura_completa=\u201dligado\u201d disabled_on=\u201ddesligado|desligado|desligado\u201d _builder_version=\u201d4.16\u2033 largura_completa_bottom=\u201d55px\u201d cor_completa_bottom=\u201d#29327a\u201d bloqueado=\u201ddesligado\u201d global_colors_info=\u201d{}\u201d][et_pb_cabe\u00e7alho_completo _builder_version=\u201d4.16\u2033 fonte_completa=\u201d||||||||\u201d title_font_size=\u201d55\u2033 background_color=\u201d#29327a\u201d background_image=\u201dhttps:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/12\/Jes.jpg\u201d background_position=\u201dcenter_left\u201d custom_padding=\u201d9vw||9vw||true\u201d custom_padding_tablet=\u201d\u201d custom_padding_phone=\u201d|56px||\u201d custom_padding_last_edited=\u201don|desktop\u201d title_font_size_tablet=\u201d45px\u201d title_font_size_phone=\u201d40px\u201d title_font_size_last_edited=\u201don|phone\u201d z_index_tablet=\u201d500\u2033 custom_css_main_element=\u201dposi\u00e7\u00e3o de fundo: centro 18% !importante;\u201d global_colors_info=\u201d{}\u201d] Protocolos de cultura de c\u00e9lulas linfobl\u00e1sticas [\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=\u201d1\u2033 use_custom_gutter=\u201don\u201d gutter_width=\u201d1\u2033 specialty=\u201don\u201d padding_left_1=\u201d35px\u201d padding_left_2=\u201d35px\u201d padding_2_tablet=\u201d|||0px\u201d padding_2_phone=\u201d\u201d padding_2_last_edited=\u201don|desktop\u201d module_class_1=\u201dsidebar-secondary-nav\u201d module_class=\u201dhandprint-bg\u201d _builder_version=\u201d4.16\u2033 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Keep cells frozen until thawed for cell culture or place in liquid nitrogen storage if not starting cultures right away. However, it is recommended that if you are going to store the cells for later use, grow up fresh stocks and freeze multiple ampoules. The cells are cryopreserved in 45% RPMI-1640, 50% fetal calf serum, and 5% DMSO (tissue culture grade).\r\n
Fax: 508-543-0377
<\/span>lgordon@progeriaresearch.org<\/a><\/p>\nWendy Norris<\/span><\/h6>\n
Telefone: 401-274-1122 x 48063
wnorris@brownhealth.org<\/a>
<\/span><\/div>\nInscrever-se<\/h2>\n
para o nosso<\/h2>\n
Atualiza\u00e7\u00f5es!<\/h2>\n
Juntos, n\u00f3s<\/h2>\n
VAI<\/em><\/h2>\n
encontre a cura!<\/h2>\n
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