{"id":8398,"date":"2020-11-02T18:57:17","date_gmt":"2020-11-02T23:57:17","guid":{"rendered":"https:\/\/www.progeriaresearch.org\/?p=8398"},"modified":"2022-12-09T06:43:20","modified_gmt":"2022-12-09T11:43:20","slug":"2020-workshop","status":"publish","type":"post","link":"https:\/\/www.progeriaresearch.org\/pt\/2020\/11\/02\/2020-workshop\/","title":{"rendered":"10\u00ba Workshop Cient\u00edfico Internacional da PRF"},"content":{"rendered":"

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Mais de 370<\/strong> registrantes de 30<\/strong> pa\u00edses participaram do nosso 1st<\/sup> sempre Reuni\u00e3o Cient\u00edfica baseada na web!<\/h4>\n

[\/et_pb_text][et_pb_image src=”https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/07\/Registrant-Map-1.jpg” title_text=”Registrant-Map” align=”center” admin_label=”map” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||20px||false|false” global_colors_info=”{}”][\/et_pb_image][et_pb_image src=”https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/12\/Complete-Photo.jpg” title_text=”Complete-Photo” align=”center” admin_label=”participants” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||15px||false|false” global_colors_info=”{}”][\/et_pb_image][et_pb_text admin_label=”video clip text” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

Esperamos que voc\u00ea goste deste clipe do nosso Workshop Internacional de 2020,
Webinar \u201cPesquisando Possibilidades, Estendendo Vidas\u201d<\/h4>\n

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\u00a0Todos os direitos reservados. Por favor, n\u00e3o baixe, copie, distribua ou modifique esta apresenta\u00e7\u00e3o de nenhuma forma.<\/i><\/p>\n

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Perguntas de bate-papo sem resposta<\/strong><\/h4>\n

[\/et_pb_text][et_pb_toggle title=”To Dr. Francis Collins: Question: From Mansoor Amiji: Dr. Collins, have you looked at the biodistribution of the PPPMO upon systemic ” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Collins Q&A” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n


\"\"Responder:<\/strong> Estudos de PK\/PD indicaram biodisponibilidade de 100% no cora\u00e7\u00e3o, aorta e rim. O rim foi o \u00fanico tecido onde houve uma sugest\u00e3o de ac\u00famulo, com base no exame histol\u00f3gico e dados de PK\/PD. A administra\u00e7\u00e3o de dose \u00fanica de SRP-2001 demonstrou que o PPMO foi rapidamente eliminado do cora\u00e7\u00e3o (dentro de 2 horas), eliminado da aorta dentro de 4 horas, mas mantido no rim at\u00e9 o ponto de tempo de 48 horas. O ddPCR quantitativo demonstrou atividade de inibi\u00e7\u00e3o de splicing cr\u00edptico G608G em todos os tecidos, exceto na pele, testados at\u00e9 agora (aorta, cora\u00e7\u00e3o, rim, f\u00edgado, quadr\u00edceps, osso), embora a atividade tenha variado dependendo do tecido.<\/p>\n

Francisco, Mike e Wayne<\/em><\/p>\n

[\/et_pb_toggle][et_pb_toggle title=”To Dr. Pinak Shah and Dr. Francesco Musumeci: Question: From RVB: Can magnesium\/calcium intake determine the degree of calcification in children with HGPS?” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Shah & Musumeci Q&A” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"<\/strong>Responder:<\/strong>Em rela\u00e7\u00e3o \u00e0 pergunta, para ser honesto, n\u00e3o sei a resposta para essa pergunta. Em um adulto t\u00edpico, no entanto, n\u00e3o correlacionamos o grau de calcifica\u00e7\u00e3o valvular com a ingest\u00e3o de Mg\/Ca, a calcifica\u00e7\u00e3o mais pesada provavelmente se deve a outros fatores.<\/p>\n

Dr. Pinak Shah<\/em><\/p>\n

 <\/p>\n

 <\/p>\n

\"\"Responder: <\/strong>Concordo com a opini\u00e3o do Dr. Shah. Provavelmente as causas da calcifica\u00e7\u00e3o da v\u00e1lvula em HGPS s\u00e3o diferentes daquelas que vemos em pacientes idosos. Tamb\u00e9m n\u00e3o tenho conhecimento sobre o metabolismo de Ca e Mg.<\/p>\n

Dr. Francesco Musumeci<\/em><\/p>\n

\u00a0<\/strong><\/p>\n

[\/et_pb_toggle][et_pb_toggle title=” To Dr. Vicente Andres: Question: From Andre Monteiro Da Rocha – University of Michigan: Do the mice lacking Lamin A develop DCM?” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Andres Q&A” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"Responder: <\/strong>O laminado A\/C nulo (Lmna<\/em>-\/-<\/sup>) modelo de camundongo desenvolve DCM acompanhado de perda da fun\u00e7\u00e3o card\u00edaca. Al\u00e9m disso, esses camundongos apresentam defeitos na condu\u00e7\u00e3o card\u00edaca e fibrose card\u00edaca extensa, e morte prematura em 5-8 semanas de idade (Auguste et al., 2018; Frock et al., 2012; Sullivan, 1999). No entanto, modelos de camundongos sem apenas Lamin A (Fong et al., 2006) ou lamin C (Coffinier et al., 2010) n\u00e3o apresentam defeitos card\u00edacos e t\u00eam uma expectativa de vida normal.<\/p>\n

Refer\u00eancias:
<\/strong>Auguste, G., Gurha, P., Lombardi, R., Coarfa, C., Willerson, JT e Marian, AJ (2018). A supress\u00e3o de fatores de transcri\u00e7\u00e3o FOXO ativados no cora\u00e7\u00e3o prolonga a sobreviv\u00eancia em um modelo murino de laminopatias<\/em>. https:\/\/doi.org\/10.1161\/CIRCRESAHA.117.312052<\/a><\/p>\n

Coffinier, C., Jung, HJ, Li, Z., Nobumori, C., Yun, UJ, Farber, EA, Davies, BS, Weinstein, MM, Yang, SH, Lammerding, J., Farahani, JN, Bentolila, LA, Fong, LG, & Young, SG (2010). A s\u00edntese direta da l\u00e2mina A, ignorando o processamento da pr\u00e9-l\u00e2mina a, causa n\u00facleos deformados em fibroblastos, mas nenhuma patologia detect\u00e1vel em camundongos. Revista de Qu\u00edmica Biol\u00f3gica<\/em>, 285<\/em>(27), 20818\u201320826. https:\/\/doi.org\/10.1074\/jbc.M110.128835<\/a><\/p>\n

Fong, LG, Bergo, MO, Young, SG, Fong, LG, Ng, JK, Lammerding, J., Vickers, TA, Meta, M., Cot\u00e9, N., Gavino, B., Qiao, X., Chang, SY, Young, SR, Yang, SH, Stewart, CL, Lee, RT, Bennett, CF, Bergo, MO, & Young, SG (2006). A pr\u00e9-l\u00e2mina A e a l\u00e2mina A parecem ser dispens\u00e1veis na l\u00e2mina nuclear. O Jornal de Investiga\u00e7\u00e3o Cl\u00ednica<\/em>, 116<\/em>(3), 743\u2013752. https:\/\/doi.org\/10.1172\/JCI27125.with<\/a><\/p>\n

Frock, RL, Chen, SC, Da, D., Frett, E., Lau, C., Brown, C., Pak, DN, Wang, Y., Muchir, A., Worman, HJ, Santana, LF, Ladiges, WC, Rabinovitch, PS, & Kennedy, BK (2012). Express\u00e3o espec\u00edfica de cardiomi\u00f3citos de lamin A melhora a fun\u00e7\u00e3o card\u00edaca em camundongos Lmna-\/-. PLoS UM<\/em>, 7<\/em>(8), 1\u20139. https:\/\/doi.org\/10.1371\/journal.pone.0042918<\/a><\/p>\n

Sullivan, T. (1999). Perda da express\u00e3o da lamina tipo A compromete a integridade do envelope nuclear, levando \u00e0 distrofia muscular. O Jornal de Biologia Celular<\/em>, 147<\/em>(5), 913\u2013920. https:\/\/doi.org\/10.1083\/jcb.147.5.913<\/a><\/p>\n

[\/et_pb_toggle][et_pb_toggle title=” To Dr. Giovanna Lattanzi: 2 Questions and Answers for Dr. Lattanzi listed below.” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Lattanzi Q&A 2″ _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"Pergunta:<\/span> <\/strong>De Tom Misteli: quanto tempo durou o tratamento com lonafarnibe + tozilizumabe em animais?
Responder:<\/span> <\/strong>Realizamos um experimento preliminar em animais heterozigotos come\u00e7ando na semana 22 at\u00e9 a semana 30. Gostar\u00edamos de nos concentrar neste per\u00edodo para avaliar o efeito no ganho de peso que foi observado anteriormente com tocilizumabe.<\/p>\n

Pergunta: <\/strong><\/span>De Sgonzalo: Linda hist\u00f3ria Giovanna e Sammy!!! Voc\u00eas viram algum efeito colateral do tratamento com tocilizumabe? Ou algum fen\u00f3tipo que n\u00e3o foi melhorado em camundongos com progeria? Para a Dra. Giovanna Lattanzi: Pergunta: De tom misteli: quanto tempo durou o tratamento com lonafarnibe + tozilizumabe em animais?
Responder: <\/strong><\/span>Obrigado Susana. N\u00e3o, n\u00e3o vimos nenhum efeito colateral do tocilizumab. Entre os fen\u00f3tipos examinados (cifose, distrofia do tecido adiposo, defeitos de cabelo e pele, atividade locomotora, les\u00f5es da aorta e hipertrofia do mioc\u00e1rdio) foram retardados\/melhorados.<\/p>\n

Coment\u00e1rio: <\/strong><\/span>De MARIE GERHARD-HERMAN: Tamb\u00e9m usado nos EUA para doen\u00e7as reumatol\u00f3gicas e vasculite
Responder:<\/span> <\/strong>Obrigada, Marie, por esta informa\u00e7\u00e3o interessante sobre o uso para vasculite.<\/p>\n

Coment\u00e1rio:<\/span> <\/strong>Do pc stefano: O tocilizumabe tamb\u00e9m \u00e9 aprovado no Jap\u00e3o para tratar a doen\u00e7a de Castleman sem efeitos imunossupressores
Responder: <\/strong><\/span>Obrigado Stefano por essas informa\u00e7\u00f5es.<\/p>\n

[\/et_pb_toggle][et_pb_toggle title=”To Dr. Minju Kim and Somi Kang who presented on behalf of Dr. Bum-Joon Park: 8 Questions and Answers for Dr. Minju Kim and Somi Kanglisted below.” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Kim and Kang Q&A 6 questions” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"\"\" <\/strong><\/p>\n

Pergunta:<\/span> <\/strong>De Delphine Larrieu: voc\u00ea v\u00ea alguma redu\u00e7\u00e3o nos danos ao DNA ap\u00f3s o tratamento com progerinina em c\u00e9lulas HGPS\/modelos de camundongos HGPS?
Responder: <\/strong><\/span>Observamos que genes relacionados ao reparo do DNA (BRCA1, Rad51, etc.) foram alterados ap\u00f3s o tratamento com progerinina.<\/p>\n

Pergunta:<\/strong> <\/span>De Delphine Larrieu: Como a progerinina funciona em Werner
S\u00edndrome que \u2013 at\u00e9 onde eu sei \u2013 n\u00e3o expressa progerina? A mol\u00e9cula tem outros alvos?
Responder: <\/strong><\/span>As c\u00e9lulas WS tamb\u00e9m podem produzir uma pequena quantidade de progerina, como acontece com as c\u00e9lulas normais envelhecidas.
c\u00e9lulas. Observamos a express\u00e3o de progerina em c\u00e9lulas WS em n\u00edveis de mRNA.
Acreditamos que a prote\u00edna WRN pode funcionar para inibir a progerina. No entanto, no
no caso de pacientes com WS, como n\u00e3o h\u00e1 prote\u00edna WRN, a express\u00e3o de progerina \u00e9
pensa-se que ocorre nas d\u00e9cadas de 20 e 30, tanto quanto \u00e9 visto em pessoas com idade normal
pessoas.<\/p>\n

Pergunta: <\/strong><\/span>De Bob Bishop: O s\u00edtio de liga\u00e7\u00e3o da progerinina \u00e9 definido bioquimicamente ou
estruturalmente? Existe um ensaio bioqu\u00edmico para encontrar compostos adicionais
com esse mecanismo?
Responder: <\/strong><\/span>Realizamos o ensaio de extra\u00e7\u00e3o de esferas de biotina-avidina e observamos a
intera\u00e7\u00e3o espec\u00edfica da perogerinina com a progerina, mas n\u00e3o com a l\u00e2mina A, l\u00e2mina
B, p53 ou emerina.
Tamb\u00e9m realizamos o ensaio Elisa para encontrar compostos adicionais que pudessem
ser supostos inibidores de liga\u00e7\u00e3o de progerina\/lamina A. A prote\u00edna recombinante de progerina foi incubada sequencialmente com uma biblioteca qu\u00edmica e l\u00e2mina A.
No entanto, a progerinina foi o produto qu\u00edmico mais eficaz na ind\u00fastria qu\u00edmica
biblioteca.\"\"<\/p>\n

\"\"<\/p>\n

Pergunta: <\/strong><\/span>De John Cooke: Grande progresso Dr. Kim. A progerinina reduz a
secre\u00e7\u00e3o de citocinas SASP?
Responder: <\/strong><\/span>Observamos que a progerinina pode reduzir a express\u00e3o de IL6 e IL8 em
C\u00e9lulas HGPS.<\/p>\n

Pergunta: <\/strong><\/span>De Susan Michaelis: a progerinina est\u00e1 dispon\u00edvel para pesquisadores?
Responder: <\/strong><\/span>Claro, est\u00e1 dispon\u00edvel se voc\u00ea solicitar.<\/p>\n

Pergunta: <\/strong><\/span>De Thomas W Glover: Posso ter perdido, mas o que voc\u00ea acha que \u00e9 o
mecanismo respons\u00e1vel pelos efeitos ben\u00e9ficos na s\u00edndrome de Werner?
Responder: <\/strong><\/span>As c\u00e9lulas derivadas de pacientes com WS tamb\u00e9m produzem uma pequena quantidade de progerina, como
tanto quanto c\u00e9lulas normais derivadas de pessoas idosas. A progerinina tamb\u00e9m pode ser
eficaz em c\u00e9lulas WS reduzindo a express\u00e3o de progerina.<\/p>\n

[\/et_pb_toggle][et_pb_toggle title=”To Dr. Martin Berg\u00f6: Question: From Mario D. Cordero: Have you studied the effect about inflammation?” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Dr. Martin Berg\u00f6” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"Responder: <\/strong>Obrigado pela pergunta: N\u00e3o, n\u00e3o \u00e9 inflama\u00e7\u00e3o em si. Parcialmente relacionado \u00e0 inflama\u00e7\u00e3o, estudamos macr\u00f3fagos neste projeto e descobrimos que o knockout de\u00a0ICM<\/i>\u00a0em aumenta o efluxo de colesterol dos macr\u00f3fagos, tanto basal quanto estimulado por ApoAI e HDL. Como esse efeito \u00e0s vezes est\u00e1 associado ao aumento do transporte reverso de colesterol in vivo, gostar\u00edamos de determinar se\u00a0ICM<\/i>\u00a0a inibi\u00e7\u00e3o reduziria o desenvolvimento da aterosclerose.<\/p>\n

[\/et_pb_toggle][et_pb_toggle title=”To Dr. David Liu: Question: 3 Questions and Answers for Dr. Lui listed below.” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Dr. David Liu Q&A 3″ _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"<\/p>\n

Pergunta: <\/strong><\/span>De vandres: voc\u00ea tentou a corre\u00e7\u00e3o de DNA em fibroblastos HGPS humanos de diferentes idades (in vitro) e testou a normaliza\u00e7\u00e3o do fen\u00f3tipo deles para ver se ele diminui \u00e0 medida que as c\u00e9lulas envelhecem?
Responder:<\/span> <\/strong>N\u00e3o, n\u00e3o tentamos corre\u00e7\u00e3o em fibroblastos de pacientes de diferentes idades \u2014 apenas de dois pacientes diferentes, que produziram resultados semelhantes.<\/p>\n

Pergunta:<\/strong> <\/span>De Delphine Larrieu: Efeitos incr\u00edveis! Voc\u00ea esperaria um KO completo de progerina em camundongos heterozigotos?
Responder:<\/span> <\/strong>No camundongo de c\u00f3pia \u00fanica, eu n\u00e3o esperaria necessariamente efici\u00eancias de edi\u00e7\u00e3o muito diferentes do que observamos atualmente, uma vez que j\u00e1 deveria haver um excesso de agente de edi\u00e7\u00e3o para cada progerina.<\/p>\n

Pergunta: <\/span><\/strong>De Andr\u00e9 Monteiro Da Rocha: Voc\u00ea acha que a reprograma\u00e7\u00e3o de uma baixa porcentagem de c\u00e9lulas pode reparar a fun\u00e7\u00e3o das c\u00e9lulas-tronco, mas pode levar \u00e0 exaust\u00e3o das c\u00e9lulas-tronco adultas?
Responder:<\/span> <\/strong>N\u00e3o temos dados que possam informar uma resposta para esta pergunta.<\/p>\n

[\/et_pb_toggle][et_pb_toggle title=”To Dr. Colin Stewart: 8 Questions and Answers for Dr. Stewart listed below.” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Dr. Colin Stewart” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

\"\"Pergunta:<\/strong><\/span> De Mei: Parece cardiomi\u00f3cito, colora\u00e7\u00e3o linda!
De Abby Buchwalter: A express\u00e3o dominante negativa de Sun1 interrompe todos os complexos LINC, incluindo aqueles mediados por Sun2?
Responder:<\/span> <\/strong>Sim, h\u00e1 evid\u00eancias disso in vitro, pois todos os Nesprins no NE podem ser deslocados pelo DNSUN1<\/p>\n

Pergunta:<\/strong><\/span> De Suet Nee Chen: como e qual \u00e9 o mecanismo pelo qual o AAV9-SunDn \u00e9 capaz de ter efeitos de longo prazo? Em que idade voc\u00ea injetou o AAV9-Sun1DN nos camundongos mutantes LMNA?
Responder:<\/span> <\/strong>Injetamos o DNSUN1 AAV um dia ap\u00f3s induzir a Cre recombinase para deletar a laminA. Temos dados de que o DNSUN1 entregue pelo AAV continua a ser expresso nos cardiomi\u00f3citos um ano ap\u00f3s a inje\u00e7\u00e3o. Estamos fazendo experimentos de dose\/tempo\/resposta agora que estamos de volta ao laborat\u00f3rio e podemos fazer nosso pr\u00f3prio AAV em quantidades suficientes<\/p>\n

Pergunta:<\/span><\/strong> De Martin Berg\u00f6, Viceordf\u00f6rande Kommitt\u00e9n f\u00f6r forskning: Qual voc\u00ea acha que \u00e9 a contribui\u00e7\u00e3o relativa da l\u00e2mina A ausente versus a l\u00e2mina C em seus experimentos de elimina\u00e7\u00e3o de Lmna?
Responder:<\/strong><\/span> N\u00e3o tenho certeza sobre isso, mas Sun1 interage com LaminA e nos camundongos HGPS a contribui\u00e7\u00e3o deve ser toda LaminA (da qual a progerina \u00e9 derivada)<\/p>\n

Pergunta:<\/span><\/strong> De jcampisi: Qual \u00e9 a causa da morte em SUN1 KO ou hets?
Responder:<\/strong><\/span> Boa pergunta, ainda n\u00e3o sabemos, mas atualmente suspeitamos de algum grupo de m\u00fasculos esquel\u00e9ticos. Precisamos fazer uma an\u00e1lise histopatol\u00f3gica decente dos ratos moribundos.<\/p>\n

Pergunta: <\/span><\/strong>De Tom Misteli: Colin: voc\u00ea viu mudan\u00e7as nas vias de resposta ao estresse celular nos animais Lmna\/Sun?
Responder:<\/strong><\/span> Ainda n\u00e3o medimos nenhum gene\/marcador de resposta ao estresse nesses camundongos<\/p>\n

Pergunta: De Delphine Larrieu: Voc\u00ea consideraria atingir apenas o cora\u00e7\u00e3o com o DNSUN1 em pacientes com HGPS?
Responder:<\/strong><\/span> Isso \u00e9 poss\u00edvel dado o sorotipo e o uso de um promotor espec\u00edfico de cardiomi\u00f3citos. Esses experimentos est\u00e3o atualmente em andamento<\/p>\n

Pergunta:<\/span><\/strong>De Yuexia Wang: Por que KO SUN1 tem menos benef\u00edcios em camundongos homozigotos G609G?
Responder:<\/strong><\/span> Presumivelmente porque os camundongos homozigotos est\u00e3o expressando mais Progerina. Vemos um efeito de resposta de heterozigotos x homozigotos em nossos experimentos gen\u00e9ticos<\/p>\n

Pergunta:<\/span><\/strong>De Roland Foisner: Ol\u00e1 Colin, boa conversa. Qual \u00e9 o efeito negativo do SUN1 regulado positivamente no HGPS. Roland
Responder:<\/strong><\/span> Provavelmente aumento da associa\u00e7\u00e3o da rede MT com o complexo LINC. Aumento da express\u00e3o de Sun\/Nesprin em c\u00e9lulas de cultura afeta o posicionamento nuclear e a migra\u00e7\u00e3o celular<\/p>\n

[\/et_pb_toggle][et_pb_image src=”https:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2020\/07\/Basso.Peraut-Family.jpg” title_text=”Basso.Peraut-Family” align=”center” admin_label=”families” _builder_version=”4.16″ _module_preset=”default” custom_margin=”5px||15px||false|false” global_colors_info=”{}”][\/et_pb_image][et_pb_toggle title=”A Sampling from the Chat Comments!” open_toggle_background_color=”#f7f7f7″ closed_toggle_text_color=”#ffffff” closed_toggle_background_color=”#00b2e2″ icon_color=”#ffc15e” open_icon_color=”#ffc15e” admin_label=”Sampling from the Chat Comments!” _builder_version=”4.16″ _module_preset=”default” title_text_color=”#00b2e2″ title_font=”||||||||” background_color=”#ffffff” custom_margin=”||25px” custom_padding=”|50px||50px||true” border_width_all=”0px” global_colors_info=”{}”]<\/p>\n

Uma honra conhecer a fam\u00edlia Basso e Peraut. Obrigado por compartilhar sua jornada. Voc\u00eas s\u00e3o verdadeiramente uma inspira\u00e7\u00e3o!<\/p>\n

Obrigada \u00e0 fam\u00edlia de Sami e Alexandra por participarem do workshop e compartilharem sua jornada inspiradora com a prog\u00e9ria!<\/p>\n

Progresso fant\u00e1stico Eiger com aprova\u00e7\u00e3o no horizonte.<\/p>\n

Podemos estender esta sess\u00e3o at\u00e9 o final da semana e continuar a discuss\u00e3o? (OBRIGADO a todos!)\u00a0<\/p>\n

Este workshop foi incr\u00edvel. Parabenizo a PRF por ter o comprometimento de fazer esta grande atividade. Sem d\u00favidas, este workshop me encheu de muito conhecimento para o meu futuro. Foi incr\u00edvel. Juntos encontraremos a cura!<\/p>\n

Sempre impressionado com a ci\u00eancia emocionante nas reuni\u00f5es do PRF. Muitos elogios aos organizadores por preservar a experi\u00eancia do workshop comunit\u00e1rio, mesmo neste formato virtual!<\/p>\n

Voc\u00eas todos ARRASAM!!! Obrigado por esta grande oportunidade, pensando em muitos anos atr\u00e1s, agora \u00e9 como viver no futuro! Feliz por fazer parte disso<\/p>\n

Sinto falta da guitarra do Dr. Collin. \u00d3timo trabalho!<\/p>\n

Muito obrigado por organizar isso, realmente o destaque da minha semana!<\/p>\n

Obrigado a todos e nos vemos pessoalmente no ano que vem em Cambridge!!<\/p>\n

\u00a0<\/strong>Obrigado a todos por se juntarem a n\u00f3s!<\/strong><\/p>\n

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Inscrever-se<\/h2>\n

para o nosso<\/h2>\n

Boletim informativo!<\/h2>\n

[\/et_pb_cta][\/et_pb_column][et_pb_column type=\u201d1_4\u2033 _builder_version=\u201d4.16\u2033 custom_padding=\u201d|||\u201d global_colors_info=\u201d{}\u201d custom_padding__hover=\u201d|||\u201d][et_pb_cta button_url=\u201dhttps:\/\/progeriaresearch.donorsupport.co\/-\/XZHJVWZR\u201d button_text=\u201dDoe agora\u201d admin_label=\u201dJuntos, encontraremos a cura!\u201d module_class=\u201dsign-btn\u201d _builder_version=\u201d4.16\u2033 header_font_size=\u201d25px\u201d background_color=\u201d#29327a\u201d animation_style=\u201dslide\u201d animation_direction=\u201dleft\u201d animation_intensity_slide=\u201d25%\u201d header_font_size_tablet=\u201d\u201d header_font_size_phone=\u201d30px\u201d header_font_size_last_edited=\u201don|desktop\u201d body_font_size_tablet=\u201d\u201d body_font_size_phone=\u201d\u201d body_font_size_last_edited=\u201don|desktop\u201d z_index_tablet=\u201d500\u2033 border_radii=\u201don|25px|25px|25px|25px\u201d global_colors_info=\u201d{}\u201d button_bg_color__hover_enabled=\u201don\u201d bot\u00e3o_bg_color__hover=\u201d#8fd2ed\u201d bot\u00e3o_border_color__hover_enabled=\u201don\u201d]<\/p>\n

Juntos, n\u00f3s<\/h2>\n

VAI<\/em><\/h2>\n

encontre a cura!<\/h2>\n

[\/et_pb_cta][\/et_pb_column][et_pb_column tipo=\u201d1_2\u2033 _builder_version=\u201d4.16\u2033 preenchimento_personalizado=\u201d|||\u201d global_colors_info=\u201d{}\u201d custom_padding__hover=\u201d|||\u201d][et_pb_image src=\u201dhttps:\/\/www.progeriaresearch.org\/wp-content\/uploads\/2022\/11\/footerstrip2022-1200-\u00d7-300-px-1.png\u201d title_text=\u201dfooterstrip2022 (1200 \u00d7 300 px) (1)\u201d _builder_version=\u201d4.18.1\u2033 _module_preset=\u201ddefault\u201d custom_margin=\u201d35px||||false|false\u201d global_colors_info=\u201d{}\u201d][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"

Aproveite alguns clipes, fotos, coment\u00e1rios e perguntas e respostas!<\/p>","protected":false},"author":2,"featured_media":8394,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[3,2],"tags":[],"class_list":["post-8398","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-events","category-news"],"yoast_head":"\nPRF\u2019s 10th International Scientific Workshop - The Progeria Research Foundation<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.progeriaresearch.org\/pt\/2020\/11\/02\/2020-workshop\/\" \/>\n<meta property=\"og:locale\" content=\"pt_PT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PRF\u2019s 10th International Scientific Workshop - 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